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Salamanders in Regeneration Research pp 91–99Cite as

Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1290))

Abstract

The realization that neuronal injury does not result in permanent functional or cellular loss in all vertebrates has fascinated regenerative biologists. Neuronal regeneration occurs in a subset of species, including lizards, teleost fish, axolotls, and newts. One tool for studying neuronal regeneration in the adult brain is intraventricular injection of selective neuronal toxins, which leads to loss of subpopulations of neurons. To trace cells involved in the regeneration process, plasmids encoding reporter proteins can be electroporated in vivo into the cells of interest. This protocol describes methods to label the ependymoglial cells of the brain of the red spotted newt Notophthalmus viridescens and follow their response after ablation of dopaminergic neurons.

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Acknowledgement

This work was supported by a grant from the Swedish Research Council to MK.

Author information

Authors and Affiliations

  1. Department of Cell and Molecular Biology, Karolinska Institutet, Berzelius väg 35, Stockholm, 171 77, Sweden

    Matthew Kirkham Ph.D. & Alberto Joven Ph.D.

Authors
  1. Matthew Kirkham Ph.D.
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  2. Alberto Joven Ph.D.
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Corresponding author

Correspondence to Matthew Kirkham Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, United Kingdom

    Anoop Kumar

  2. Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden

    András Simon

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Kirkham, M., Joven, A. (2015). Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation. In: Kumar, A., Simon, A. (eds) Salamanders in Regeneration Research. Methods in Molecular Biology, vol 1290. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2495-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-2495-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2494-3

  • Online ISBN: 978-1-4939-2495-0

  • eBook Packages: Springer Protocols

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