Abstract
The use of cells grown in vitro has been instrumental for multiple aspects of biomedical research and especially molecular and cellular biology. The ability to grow cells from multicellular organisms like humans, squids, or salamanders is important to simplify the analyses and experimental designs to help understand the biology of these organisms. The advent of the first cell culture has allowed scientists to tease apart the cellular functions, and in many situations these experiments help understand what is happening in the whole organism. In this chapter, we describe techniques for the culture and genetic manipulation of an established cell line from axolotl, a species widely used for studying epimorphic regeneration.
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References
Roy S, Gardiner DM, Bryant SV (2000) Vaccinia as a tool for functional analysis in regenerating limbs: ectopic expression of Shh. Dev Biol 218:199–205
Levesque M, Gatien S, Finnson K, Desmeules S, Villiard E, Pilote M, Philip A, Roy S (2007) Transforming growth factor: beta signaling is essential for limb regeneration in axolotls. PLoS One 2:e1227. doi:10.1371/journal.pone.0001227
Shaikh N, Gates PB, Brockes JP (2011) The Meis homeoprotein regulates the axolotl Prod 1 promoter during limb regeneration. Gene 484:69–74
Villiard E, Brinkmann H, Moiseeva O, Mallette FA, Ferbeyre G, Roy S (2007) Urodele p53 tolerates amino acid changes found in p53 variants linked to human cancer. BMC Evol Biol 7:180. doi:10.1186/1471-2148-7-180, 1471-2148-7-180 [pii]
Guimond JC, Levesque M, Michaud PL, Berdugo J, Finnson K, Philip A, Roy S (2010) BMP-2 functions independently of SHH signaling and triggers cell condensation and apoptosis in regenerating axolotl limbs. BMC Dev Biol 10:15. doi:10.1186/1471-213X-10-15
Whited JL, Tsai SL, Beier KT, White JN, Piekarski N, Hanken J, Cepko CL, Tabin CJ (2013) Pseudotyped retroviruses for infecting axolotl in vivo and in vitro. Development 140:1137–1146
Moore D, Dowhan D (2002) Purification and concentration of DNA from aqueous solutions. Curr Protoc Mol Biol, Chapter 2:Unit 2.1A. doi: 10.1002/0471142727.mb0201as59. John Wiley & Sons, Inc.
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Denis, JF., Sader, F., Ferretti, P., Roy, S. (2015). Culture and Transfection of Axolotl Cells. In: Kumar, A., Simon, A. (eds) Salamanders in Regeneration Research. Methods in Molecular Biology, vol 1290. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2495-0_15
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DOI: https://doi.org/10.1007/978-1-4939-2495-0_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2495-0
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