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Salamanders in Regeneration Research pp 147–157Cite as

Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1290))

Abstract

Limb regeneration of salamanders is nerve dependent, and the removal of the nerves in early stages of limb regeneration severely curtails the proliferation of the blastemal cells and growth of the regenerate. The removal of the neural tube from a developing salamander embryo results in an aneurogenic larva and the aneurogenic limb (ANL) develops independently without innervation. Paradoxically, the limb in an ANL is capable of regeneration in a nerve-independent manner. Here, we describe a detailed method for the generation of ANL in the spotted salamander, Ambystoma maculatum, for regeneration studies.

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Acknowledgements

We thank Professor Jeremy Brockes for advice and support. Our work is funded by a Program Grant and MRC Research Professorship to Jeremy Brockes. JP Delgado is funded by Programa-Sostenibilidad 2013–2014, University of Antioquia, Colombia.

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Authors and Affiliations

  1. Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, WC1E 6BT, UK

    Anoop Kumar

  2. Grupo Genética, Regeneración & Cáncer, Instituto de Biología, Universidad de Antioquia, Calle 67 NO. 53-108, Medellin, Colombia

    Jean Paul Delgado Ph.D.

Authors
  1. Anoop Kumar
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  2. Jean Paul Delgado Ph.D.
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Corresponding author

Correspondence to Anoop Kumar .

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Editors and Affiliations

  1. Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, United Kingdom

    Anoop Kumar

  2. Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden

    András Simon

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Kumar, A., Delgado, J.P. (2015). Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos. In: Kumar, A., Simon, A. (eds) Salamanders in Regeneration Research. Methods in Molecular Biology, vol 1290. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2495-0_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2495-0_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2494-3

  • Online ISBN: 978-1-4939-2495-0

  • eBook Packages: Springer Protocols

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