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Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support

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Chromatin Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1288))

Abstract

Complex experimental strategies involving in vitro reconstituted chromatin or simple chromatin interaction studies are much facilitated by immobilizing the nucleosomal arrays to paramagnetic beads. Chromatin-containing beads can be retrieved from a reaction mix solution on a magnet fast and quantitatively, effectively separating bound, loosely attached and unbound components efficiently. This chapter details a convenient strategy for immobilization of linear plasmid DNA on streptavidin-coated beads, the reconstitution of chromatin on such beads, and some fundamental handling procedures.

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Author information

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus, Alexandroupolis, 68100, Greece

    Raphael Sandaltzopoulos

  2. Molékularbiologie, Adolf-Butenandt-Institut Ludwig-Maximilians-Universität München (LMU), Munich, Germany

    Peter B. Becker

Authors
  1. Raphael Sandaltzopoulos
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  2. Peter B. Becker
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Corresponding author

Correspondence to Raphael Sandaltzopoulos .

Editor information

Editors and Affiliations

  1. H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida, USA

    Srikumar P. Chellappan

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Sandaltzopoulos, R., Becker, P.B. (2015). Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support. In: Chellappan, S. (eds) Chromatin Protocols. Methods in Molecular Biology, vol 1288. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2474-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2474-5_1

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2473-8

  • Online ISBN: 978-1-4939-2474-5

  • eBook Packages: Springer Protocols

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