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Tol2-Mediated Transgenesis and Its Application to Electroporation

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Electroporation Methods in Neuroscience

Part of the book series: Neuromethods ((NM,volume 102))

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Abstract

The Tol2 element is an active transposon that was found from the genome of the Japanese medaka fish. Since the Tol2 transposition system is active in any vertebrate cells so far tested, it has been applied to germ-line transgenesis in various model animals including fish, frog, chicken, and mouse, and also gene transfer to culture cells. Recently, since the Tol2 system can enhance integration of exogenous DNAs into the genome, it has been successfully applied to transgene delivery to somatic cells in chicken and mouse embryos by means of in ovo and in utero electroporation, respectively, providing novel approaches to study cell differentiation and cell lineages.

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Authors and Affiliations

  1. Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, 411-8540, Japan

    Koichi Kawakami

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  1. Koichi Kawakami
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Correspondence to Koichi Kawakami .

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Editors and Affiliations

  1. Department of Developmental Biology, Chiba University, Graduate School of Medicine, Chiba, Japan

    Tetsuichiro Saito

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Kawakami, K. (2015). Tol2-Mediated Transgenesis and Its Application to Electroporation. In: Saito, T. (eds) Electroporation Methods in Neuroscience. Neuromethods, vol 102. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2459-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2459-2_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2458-5

  • Online ISBN: 978-1-4939-2459-2

  • eBook Packages: Springer Protocols

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