Abstract
Consistent and efficient gene transfer into postmitotic neurons is desirable for the study of neurons. Recent refinement of cell culturing techniques has enabled us to prepare various types of neurons in dissociated cultures. Among them, hippocampal neurons rapidly polarize, form complex morphology after plating, and are of great use in many studies. Although progenitors of hippocampal neurons can be efficiently transfected by in utero electroporation, postmitotic neurons are less susceptible to transfection than progenitors. We have developed an in vitro electroporation method, which allows for quick and easy transfection into dissociated hippocampal neurons.
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Acknowledgements
We thank all the members of our laboratory for their helpful comments. This work was supported by JSPS KAKENHI Grant Numbers 24300116, 25123701, and 26640026 to T.S. and 26460269 to A.B.
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Baba, A., Saito, T. (2015). Electroporation of Dissociated Hippocampal Neurons. In: Saito, T. (eds) Electroporation Methods in Neuroscience. Neuromethods, vol 102. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2459-2_13
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DOI: https://doi.org/10.1007/978-1-4939-2459-2_13
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