Abstract
To facilitate cotton functional genomics studies and identify cotton genes mediating biotic and abiotic stress responses, we report here a detailed protocol of construction of a VIGS library from diploid cotton Gossypium raimondii. Sequencing of the representative colonies revealed that the library covers a significant percentage of unique cotton genes. Based on the number of colonies in the primary library, it is estimated that this VIGS library is about 50× coverage of predicted cotton protein-coding genes. As a proof-of-concept experiment, we further describe a protocol to identify the genetic determinants involved in cotton drought tolerance using this VIGS library. The cotton VIGS library established here provides a unique opportunity to identify essential cotton genes in various stress responses and developmental growth regulation.
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Acknowledgments
The cotton work in He’s lab is supported by USDA NIFA (2012-67013-19433). F. L. was supported by the China Scholarship Council.
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Li, M., Li, F., He, P. (2015). Construction of a Cotton VIGS Library for Functional Genomics Study. In: Mysore, K., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 1287. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2453-0_20
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DOI: https://doi.org/10.1007/978-1-4939-2453-0_20
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Publisher Name: Humana Press, New York, NY
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