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Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using Rice tungro bacilliform virus (RTBV) as a Vector

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Plant Gene Silencing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1287))

Abstract

The large-scale functional analysis of genes in plants depends heavily on robust techniques for gene silencing. Virus-induced gene silencing (VIGS) is a transient gene silencing method for plants, triggered by the inoculation of a modified viral vector carrying a fragment of the gene targeted for silencing. Here we describe a VIGS protocol for rice, based on the Rice tungro bacilliform virus (RTBV, a DNA virus). We present an updated and detailed protocol for silencing of the gene encoding Phytoene desaturase in rice, using the RTBV-VIGS system.

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Acknowledgment

Ravi Kant and Shweta Sharma are thankful to the Indian Council of Medical Research and Council of Scientific and Industrial Research, Government of India, respectively, for research fellowships. Research in the lab of Indranil Dasgupta is supported by the Department of Biotechnology, Government of India and University of Delhi.

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Authors and Affiliations

  1. Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Ravi Kant, Shweta Sharma & Indranil Dasgupta

Authors
  1. Ravi Kant
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  2. Shweta Sharma
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  3. Indranil Dasgupta
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Corresponding author

Correspondence to Indranil Dasgupta .

Editor information

Editors and Affiliations

  1. Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA

    Kirankumar S. Mysore

  2. National Institute of Plant Genome Research, New Delhi, Delhi, India

    Muthappa Senthil-Kumar

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Kant, R., Sharma, S., Dasgupta, I. (2015). Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using Rice tungro bacilliform virus (RTBV) as a Vector. In: Mysore, K., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 1287. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2453-0_15

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  • DOI: https://doi.org/10.1007/978-1-4939-2453-0_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2452-3

  • Online ISBN: 978-1-4939-2453-0

  • eBook Packages: Springer Protocols

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