Abstract
Cryogels, by their particular morphology and mechanical properties, proved to be invaluable materials in biomedicine and biotechnology as carriers for molecules and cells, chromatographic materials for cell separations and cell culture. Methods used in the characterization of porosity and sorption properties of cryogels are very needful tools, which assist the investigator in the decision on the performances of the gel. Herein, we describe the preparation of ionic interpenetrating polymer network composite cryogels and the characterization methods of their porous morphology, and then the methods used for testing their sorption properties for ionic dyes used as models for drugs.
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Zeng X, Wei W, Li X, Zeng J, Wu L (2007) Direct electrochemistry and electrocatalysis of hemoglobin entrapped in semi-interpenetrating polymer network hydrogel based on polyacrylamide and chitosan. Bioelectrochemistry 71:135–141
Liang S, Liu L, Huang Q, Yam KL (2009) Preparation of single or double-network chitosan/poly(vinyl alcohol) gel films through selectively cross-linking method. Carbohydr Polym 77:718–724
Dragan ES, Apopei DF (2011) Synthesis and swelling behavior of pH-sensitive semi-interpenetrating polymer network composite hydrogels based on native and modified potatoes starch as potential sorbent for cationic dyes. Chem Eng J 178:252–263
Dragan ES, Perju MM, Dinu MV (2012) Preparation and characterization of IPN composite hydrogels based on polyacrylamide and chitosan and their interaction with ionic dyes. Carbohydr Polym 88:270–281
Lozinsky VL, Plieva FM, Galaev IY, Mattiasson B (2001) The potential of polymeric cryogels in bioseparation. Bioseparation 10:163–188
Bajpai AK, Shukla SK, Bhanu S, Kankane S (2008) Responsive polymers in controlled drug delivery. Prog Polym Sci 33:1088–1118
Peppas NA, Hilt JZ, Khademhosseini A, Langer R (2006) Hydrogels in biology and medicine: from molecular principles to bionanotechnology. Adv Mater 18:1345–1360
Savina IN, Cnudde V, D’Hollander S, Van Hoorebeke L, Mattiasson B, Galaev IY, Du Prez F (2007) Cryogels from poly(hydroxyethyl methacrylate): macroporous, interconnected materials with potential as cell scaffolds. Soft Matter 3:1176–1184
Baydemir G, Bereli N, Andac M, Say R, Galaev IY, Denizli A (2009) Bilirubin recognition via molecularly imprinted supermacroporous cryogels. Colloids Surf B Biointerfaces 68:33–38
Kathuria N, Tripathi A, Kar KK, Kumar A (2009) Synthesis and characterization of elastic and macroporous chitosan–gelatin cryogels for tissue engineering. Acta Biomater 5:406–418
Dispinar T, Van Camp W, De Cock LJ, De Geest BG, Du Prez FE (2012) Redox-responsive degradable PEG cryogels as potential cell scaffolds in tissue engineering. Macromol Biosci 12:383–394
Dinu MV, Ozmen MM, Dragan ES, Okay O (2007) Freezing as a path to build macroporous structures: superfast responsive polyacrylamide hydrogels. Polymer 48:195–204
Dinu MV, Perju MM, Dragan ES (2011) Porous semi-interpenetrating hydrogel networks based on dextran and polyacrylamide with superfast responsiveness. Macromol Chem Phys 212:240–251
Dinu MV, Perju MM, Dragan ES (2011) Composite IPN ionic hydrogels based on polyacrylamide and dextran sulfate. React Funct Polym 71:881–890
Burova TV, Grinberg NV, Kalinina EV, Ivanov RV, Lozinsky VI, Lorenzo CA, Grinberg VY (2011) Thermoresponsive copolymer cryogel possessing molecular memory: synthesis, energetics of collapse and interaction with ligands. Macromol Chem Phys 212:72–80
Kirsebom H, Topggard D, Galaev IY, Mattiasson B (2010) Modulating the porosity of cryogels by influencing the nonfrozen liquid phase through the addition of inert solutes. Langmuir 26:16129–16133
Jain E, Karande AA, Kumar A (2011) Supermacroporous polymer-based cryogel bioreactor for monoclonal antibody production in continuous culture using hybridoma cells. Biotechnol Prog 27:170–180
Tekin K, Uzun L, Şahin CA, Bektaş S, Denizli A (2011) Preparation and characterization of composite cryogels containing imidazole group and use in heavy metal removal. React Funct Polym 71:985–993
Liu M, Liu H, Bai L, Liu Y, Cheng J, Yang G (2011) Temperature swing adsorption of melamine on thermosensitive poly(N-isopropylacrylamide) cryogels. J Mater Sci 46:4820–4825
Hajizadeh S, Kirsebom H, Galaev IY, Mattiasson B (2010) Evaluation of selective composite cryogel for bromate removal from drinking water. J Sep Sci 33:1752–1759
Demiryas N, Tuzmen N, Galaev IY, Pişkin E, Denizli A (2007) Poly(acrylamide-allyl glycidyl ether) cryogel as a stationary phase in dye affinity chromatography. J Appl Polym Sci 105:1808–1816
Uygun DA, Akduman B, Uygun M, Akgöl S, Denizli A (2012) Purification of papain using Reactive Green 5 attached supermacroporous monolithic cryogel. Appl Biochem Biotechnol 167:552–563
Dragan ES, Lazar MM, Dinu MV, Doroftei F (2012) Macroporous composite IPN hydrogels based on poly(acrylamide) and chitosan with tuned swelling and sorption of cationic dyes. Chem Eng J 204–206:198–209
Dragan ES, Apopei Loghin DF (2013) Enhanced sorption of Methylene Blue from aqueous solutions by semi-IPN composite cryogels with anionically modified potato starch entrapped in PAAm matrix. Chem Eng J 234:211–222
Dragan ES (2014) Design and applications of interpenetrating polymer network hydrogels. A review. Chem Eng J 243:572–590
Risbud MV, Bhonde RR (2000) Polyacrylamide-chitosan hydrogels: in vitro biocompatibility and sustained antibiotic release studies. Drug Deliv 7:69–75
Ekici S, Saraydin D (2004) Synthesis, characterization and evaluation of IPN hydrogels for antibiotic release. Drug Deliv 11:381–388
Rinaudo M (2008) Main properties and current applications of some polysaccharides as biomaterials. Polym Int 57:397–430
Xia YQ, Guo TY, Song MD, Zhang BH, Zhang BL (2005) Hemoglobin recognition by imprinting in semi-interpenetrating polymer network hydrogel based on polyacrylamide and chitosan. Biomacromolecules 6:2601–2606
Gerente C, Lee VKC, Le Cloirec P, McKay G (2007) Application of chitosan for the removal of metals from wastewaters by adsorption—mechanisms and models review. Crit Rev Environ Sci Tech 37:41–127
Keshava Murthy PS, Murali Mohan Y, Sreeramulu J, Mohana Raju K (2006) Semi-IPNs of starch and poly(acrylamide-co-sodium methacrylate): Preparation, swelling and diffusion characteristics evaluation. React Funct Polym 66:1482–1493
Reis AV, Guilherme MR, Moia TA, Mattoso LHC, Muniz EC, Tambourgi EB (2008) Synthesis and characterization of a starch- modified hydrogel as potential carrier for drug delivery system. J Polym Sci A Polym Chem 46:2567–2574
Dragan ES, Apopei Loghin DF (2013) Multiresponsive macroporous semi-IPN composite hydrogels based on native or anionically modified potato starch. Carbohydr Polym 92:23–32
Dinu MV, Pradny M, Dragan ES, Michalek J (2013) Ice-templated hydrogels based on chitosan with tailored porous morphology. Carbohydr Polym 94:170–178
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Dragan, E.S., Dinu, M.V. (2015). Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption Properties. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_20
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DOI: https://doi.org/10.1007/978-1-4939-2447-9_20
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2446-2
Online ISBN: 978-1-4939-2447-9
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