Skip to main content
SpringerLink
Log in
Book cover

Affinity Chromatography pp 233–237Cite as

Molecularly Imprinted Cryogels for Human Serum Albumin Depletion

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1286))

Abstract

Molecularly imprinted polymers can be used for the selective capture of a target molecule from complex medium. Cryogels novel matrices, which characterized by their supermacropores that makes their use advantageous when studying with biological samples. By combining high selectivity of the molecular imprinting approach with using cryogel as a base polymer, in this protocol, preparation of the albumin-imprinted cryogels is described. This material is a useful candidate for the selective albumin depletion from the human serum sample prior to the detailed proteomic analysis.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Roche S, Tiers L, Provansal M, Seveno M, Piva MT, Jouin P, Lehmann S (2009) Depletion of one, six, twelve or twenty major blood proteins before proteomic analysis: the more the better? J Proteomics 72:945–951

    Article  CAS  PubMed  Google Scholar 

  2. Granger J, Siddiqui J, Copeland S, Remick D (2005) Albumin depletion of human plasma also removes low abundance proteins including the cytokines. Proteomics 5:4713–4718

    Article  CAS  PubMed  Google Scholar 

  3. Yocum AK, Yu K, Oe T, Blair IA (2005) Effect of immunoaffinity depletion of human serum during proteomic investigations. J Proteome Res 4:1722–1731

    Article  CAS  PubMed  Google Scholar 

  4. Chen YY, Lin SY, Yeh YY, Hsiao HH, Wu CY, Chen ST, Wang AHJ (2005) A modified protein precipitation procedure for efficient removal of albumin from serum. Electrophoresis 26:2117–2127

    Article  CAS  PubMed  Google Scholar 

  5. Andac M, Galaev I, Denizli A (2012) Dye attached poly(hydroxyethyl methacrylate) cryogel for albumin depletion from human serum. J Sep Sci 35:1173–1182

    Article  CAS  PubMed  Google Scholar 

  6. Urbas L, Brne P, Gabor B, Barut M, Stirlic M, Petric TC, Strancar A (2009) Depletion of high abundance proteins from human plasma using a combination of an affinity and pseudo affinity column. J Chromatogr A 1216:2689–2694

    Article  CAS  PubMed  Google Scholar 

  7. Altıntaş EB, Denizli A (2006) Efficient removal of albumin from human serum by monosize dye affinity beads. J Chromatogr B 832:216–223

    Article  Google Scholar 

  8. Wang YY, Cheng P, Chan DW (2003) A simple affinity spin tube filter method for removing high-abundant common proteins or enriching low-abundant biomarkers for serum proteomic analysis. Proteomics 3:243–248

    Article  CAS  PubMed  Google Scholar 

  9. Sato AK, Sexton DJ, Morganelli LA, Cohen EH, Wu QL, Conley GP, Streltsova Z, Lee SW, Devlin M, De Oliveira DB, Enright J, Kent RB, Wescott CR, Ransohoff TC, Ley AC, Ladner RC (2002) Development of mammalian serum albumin affinity purification media by peptide phage display. Biotechnol Prog 18:182–192

    Article  CAS  PubMed  Google Scholar 

  10. Altıntaş EB, Tüzmen N, Uzun L, Denizli A (2007) Immobilized metal affinity adsorption for antibody depletion from human serum with monosize beads. Ind Eng Chem Res 46:7802–7810

    Article  Google Scholar 

  11. Karataş M, Akgöl S, Yavuz H, Say R, Denizli A (2007) Immunoglobulin G depletion from human serum with metal chelated beads under magnetic field. Int J Biol Macromol 40:254–260

    Article  PubMed  Google Scholar 

  12. Bereli N, Şener G, Altıntaş EB, Yavuz H, Denizli A (2010) Poly(glycidyl methacrylate) beads embedded cryogels for pseudospecific affinity depletion of albumin and immunoglobulin G. Mater Sci Eng C 30:323–329

    Article  CAS  Google Scholar 

  13. Mosbach K (1994) Molecular imprinting. Trends Biochem Sci 19:9–14

    Article  CAS  PubMed  Google Scholar 

  14. Lanza F, Sellergren B (2001) Molecularly imprinted extraction materials for highly selective sample cleanup and analyte enrichment. Adv Chromatogr 3:41137–41173

    Google Scholar 

  15. Chianella I, Piletsky SA, Tothill IE, Chen B, Turner APF (2003) MIP-based solid phase extraction cartridges combined with MIP-based sensors for the detection of microcystin-LR. Biosens Bioelectron 18:119–127

    Article  CAS  PubMed  Google Scholar 

  16. Andac M, Say R, Denizli A (2004) Molecular recognition based cadmium removal from human plasma. J Chromatogr B 811:119–126

    Article  CAS  Google Scholar 

  17. Wulff G (1995) Molecular imprinting in cross-linked materials with the aid of molecular templates. Angew Chem Int Ed Engl 34:1812–1832

    Article  CAS  Google Scholar 

  18. Schirhagl R, Podlipna D, Lieberzeit PA, Dickert FL (2010) Comparing biomimetic and biological receptors for insulin sensing. Chem Commun 46:3128–3130

    Article  CAS  Google Scholar 

  19. Wangchareansak T, Sangma C, Choowongkomon K, Dickert FL, Lieberzeit PA (2011) Surface molecular imprints of WGA lectin as artificial receptors for mass-sensitive binding studies. Anal Bioanal Chem 400:2499–2506

    Article  CAS  PubMed  Google Scholar 

  20. Turner NW, Jeans CW, Brain KR, Allender CJ, Hlady V, Britt DW (2006) From 3D to 2D: a review of the molecular imprinting of proteins. Biotechnol Prog 22:1474–1489

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Bereli N, Andaç M, Baydemir G, Say R, Galaev IY, Denizli A (2008) Protein recognition via ion-coordinated moleculary imprinted supermacroporous cryogels. J Chromatogr A 1190:18–26

    Article  CAS  PubMed  Google Scholar 

  22. Andac M, Galaev IY, Denizli A (2013) Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum. Colloids Surf B: Biointerfaces 109:259–265

    Article  CAS  PubMed  Google Scholar 

  23. Tamahkar E, Bereli N, Say R, Denizli A (2011) Molecularly imprinted supermacroporous cryogels for cytochrome c recognition. J Sep Sci 34:3433–3440

    Article  CAS  PubMed  Google Scholar 

  24. Aslıyüce S, Uzun L, Rad AY, Unal S, Say R, Denizli A (2012) Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci 889–890:95–102

    Article  PubMed  Google Scholar 

  25. Bereli N, Saylan Y, Uzun L, Say R, Denizli A (2011) L-histidine imprinted supermacroporous cryogels for protein recognition. Sep Purif Technol 82:28–35

    Article  CAS  Google Scholar 

  26. Bereli N, Ertürk G, Tümer MA, Say R, Denizli A (2013) Oriented immobilized anti-IgG via Fc fragment-imprinted PHEMA cryogel for IgG purification. Biomed Chromatogr 27:599–607

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry Division, Department of Chemistry, Hacettepe University, Chemistry Department, Ankara, 06800, Turkey

    Muge Andac, Handan Yavuz & Adil Denizli

  2. DSM Nutritional Products, DBC, Delft, The Netherlands

    Igor Yu Galaev

Authors
  1. Muge Andac
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Igor Yu Galaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Handan Yavuz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Adil Denizli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adil Denizli .

Editor information

Editors and Affiliations

  1. Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany

    Senta Reichelt

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Andac, M., Galaev, I.Y., Yavuz, H., Denizli, A. (2015). Molecularly Imprinted Cryogels for Human Serum Albumin Depletion. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_19

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-2447-9_19

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2446-2

  • Online ISBN: 978-1-4939-2447-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation