Abstract
Boronate affinity monolithic columns have been developed into an important means for the selective recognition and capture of cis-diol-containing biomolecules, such as glycoproteins, nucleosides and saccharides. The ligands of boronic acids are playing an important role in boronate affinity monolithic columns. Although several boronate affinity monoliths with high affinity toward cis-diol-containing biomolecules have been reported, only few publications are focused on their detailed procedures for preparation and characterization. This chapter describes in detail the preparation and characterization of a boronate affinity monolithic column applying 2,4-difluoro-3-formyl-phenylboronic acid (DFFPBA) as a ligand. The DFFPBA-functionalized monolithic column not only exhibited an ultrahigh boronate affinity toward cis-diol-containing biomolecules, but also showed great potential for the selective enrichment of cis-diol-containing biomolecules in real samples.
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Acknowledgement
We gratefully acknowledge the financial support from the National Natural Science Foundation of China (grants nos. 21075063, 21275073, and 21121091), the Natural Science Foundation of Jiangsu Province (grant no. KB2011054), and the Ministry of Science and Technology of China (grant no. 35-15).
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Li, Q., Liu, Z. (2015). Preparation and Characterization of Fluorophenylboronic Acid-Functionalized Affinity Monolithic Columns for the Selective Enrichment of cis-Diol-Containing Biomolecules. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_13
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DOI: https://doi.org/10.1007/978-1-4939-2447-9_13
Publisher Name: Humana Press, New York, NY
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