Abstract
This chapter details procedures for (1) functionalizing macroporous silica particles with lectins, a class of proteins that have affinity for the glycan moieties on glycoproteins, and (2) utilizing the lectin-silica material for high-performance affinity chromatography (HPAC) to enrich glycoproteins from small volumes of biological sample materials.
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References
Lis H, Sharon N, Katchals E (1969) Identification of carbohydrate-protein linking group in soybean hemagglutinin. Biochim Biophys Acta 192:364–366
Muller AJ, Carr PW (1986) Examination of the thermodynamic and kinetic characteristics of microparticulate affinity-chromatographysupports—Application to Concanavalin-A. J Chromatogr 357:11–32
Madera M, Mechref Y, Novotny MV (2005) Combining lectin microcolumns with high-resolution separation techniques for enrichment of glycoproteins and glycopeptides. Anal Chem 77:4081–4090
Mann BF, Mann AKP, Skrabalak SE, Novotny MV (2013) Sub 2-mu m macroporous silica particles derivatized for enhanced lectin affinity enrichment of glycoproteins. Anal Chem 85:1905–1912
Mann BF, Goetz JA, House MG, Schmidt CM, Novotny MV (2012) Glycomic and proteomic profiling of pancreatic cyst fluids identifies hyperfucosylated lactosamines on the N-linked glycans of overexpressed glycoproteins. Mol Cell Proteomics 11:M1111.015792
Larsson P-O, Glad M, Hansson L, Månsson MO, Ohlson S, Mosbach K (1983) Advances in Chromatography. Dekker, New York
Gestwicki JE, Cairo CW, Strong LE, Oetjen KA, Kiessling LL (2002) Influencing receptor-ligand binding mechanisms with multivalent ligand architecture. J Am Chem Soc 124:14922–14933
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Mann, B.F. (2015). Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of Glycoproteins. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_10
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DOI: https://doi.org/10.1007/978-1-4939-2447-9_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2446-2
Online ISBN: 978-1-4939-2447-9
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