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Affinity Chromatography: A Historical Perspective

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Book cover Affinity Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1286))

Abstract

Affinity chromatography is one of the most selective and versatile forms of liquid chromatography for the separation or analysis of chemicals in complex mixtures. This method makes use of a biologically related agent as the stationary phase, which provides an affinity column with the ability to bind selectively and reversibly to a given target in a sample. This review examines the early work in this method and various developments that have lead to the current status of this technique. The general principles of affinity chromatography are briefly described as part of this discussion. Past and recent efforts in the generation of new binding agents, supports, and immobilization methods for this method are considered. Various applications of affinity chromatography are also summarized, as well as the influence this field has played in the creation of other affinity-based separation or analysis methods.

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Acknowledgements

This work was supported, in part, by the National Institutes of Health under grants R01 DK069629 and R01 GM044931 and by the National Science Foundation under grants CMI 1309806 and EPS 1004094.

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  1. Department of Chemistry, University of Nebraska-Lincoln, 704 Hamilton Hall, 639 N 12 Street, Lincoln, NE, 68588-0304, USA

    David S. Hage & Ryan Matsuda

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  1. David S. Hage
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Correspondence to David S. Hage .

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  1. Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany

    Senta Reichelt

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Hage, D.S., Matsuda, R. (2015). Affinity Chromatography: A Historical Perspective. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_1

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