Abstract
Genome-wide association (GWA) mapping is a powerful technique to address the molecular basis of genotype to phenotype relationships and to map regulators of biological processes. This chapter presents a protocol for genome-wide association mapping in Arabidopsis thaliana using the user-friendly internet application GWAPP, and provides a specific protocol for acquiring root trait data suitable for GWA studies using the semi-automated, high-throughput phenotyping pipeline BRAT for early root growth.
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Acknowledgement
We wish to acknowledge all those involved in the development and optimization of the wet laboratory methods used in this article: Bonnie Wohlrab, Takehiko Ogura, and Santosh Satbhai. Thanks to Takehiko Ogura for his constructive suggestions for the scanning support frame. We also thank Bjarni Vilhjalmsson and Arthur Korte for discussions regarding GWAS. We are grateful to members of the Busch lab for critically reading this protocol and Thomas Friese for editing.
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Slovak, R., Göschl, C., Seren, Ü., Busch, W. (2015). Genome-Wide Association Mapping in Plants Exemplified for Root Growth in Arabidopsis thaliana . In: Alonso, J., Stepanova, A. (eds) Plant Functional Genomics. Methods in Molecular Biology, vol 1284. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2444-8_17
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DOI: https://doi.org/10.1007/978-1-4939-2444-8_17
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2444-8
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