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Sedimentation Equilibrium Studies

  • Protocol
Protein-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1278))

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Abstract

The reversible formation of protein-protein interactions plays a crucial role in many biological processes. In order to carry out a thorough quantitative characterization of these interactions it is essential to establish the oligomerization state of the individual components first. The sedimentation equilibrium method is ideally suited to perform these studies because it allows a reliable, accurate, and absolute value of the solution molecular weight of a macromolecule to be obtained. This technique is independent of the shape of the macromolecule under investigation and allows the determination of equilibrium constants for a monomer–multimer self-associating system.

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Acknowledgement

This work was supported by the Medical Research Council, UK.

Author information

Authors and Affiliations

  1. Division of Molecular Structure, MRC National Institute for Medical Research, The Ridgeway Mill Hill, London, NW7 1AA, UK

    Ian A. Taylor & Katrin Rittinger

  2. Division of Physical Biochemistry, MRC National Institute for Medical Research, London, UK

    John F. Eccleston

Authors
  1. Ian A. Taylor
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  2. Katrin Rittinger
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  3. John F. Eccleston
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Corresponding author

Correspondence to Katrin Rittinger .

Editor information

Editors and Affiliations

  1. Department of Pharmacology and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, Georgia, USA

    Cheryl L. Meyerkord

  2. Department of Pharmacology, Department of Hematology & Medical Oncology, and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, USA

    Haian Fu

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Taylor, I.A., Rittinger, K., Eccleston, J.F. (2015). Sedimentation Equilibrium Studies. In: Meyerkord, C., Fu, H. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 1278. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2425-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2425-7_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2424-0

  • Online ISBN: 978-1-4939-2425-7

  • eBook Packages: Springer Protocols

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