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Gene Essentiality pp 1–14Cite as

Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1279))

Abstract

Although whole genome approaches to the study of bacteria have grown substantially in the past decade, there is still a need for quick and easy methods for the determination of which genes are essential for the growth of these bacteria under specific growth conditions. There are numerous methods to accomplish this depending on the resources and equipment available, each with their own advantages and disadvantages. Here we describe a method we successfully employed to map the essential genes of Campylobacter jejuni using a microarray transposon tracking approach where we constructed a saturated transposon mutant library in the C. jejuni strain NCTC11168 and used a genomic microarray approach to identify genes lacking transposon insertions under standard laboratory growth conditions. With a fully saturated library, the absence of transposon insertions can be used as an indicator of a gene essential for the survival and growth for the conditions used for the mutant library.

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Author information

Authors and Affiliations

  1. Division of Gastroenterology, BC’s Children’s Hospital, the Child and Family Research Institute and the University of British Columbia, Vancouver, BC, Canada

    Martin Stahl

  2. Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, Ottawa Institute of Systems Biology, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada, K1H 8M5

    Martin Stahl & Alain Stintzi

Authors
  1. Martin Stahl
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  2. Alain Stintzi
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Correspondence to Alain Stintzi .

Editor information

Editors and Affiliations

  1. Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio, USA

    Long Jason Lu

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Stahl, M., Stintzi, A. (2015). Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni . In: Lu, L. (eds) Gene Essentiality. Methods in Molecular Biology, vol 1279. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2398-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2398-4_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2397-7

  • Online ISBN: 978-1-4939-2398-4

  • eBook Packages: Springer Protocols

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