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Designing Primers for SNaPshot Technique

  • Greiciane Gaburro PanetoEmail author
  • Francisco de Paula Careta
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1275)

Abstract

The SNaPshot technique, also known as minisequencing, is a primer extension-based method developed for the analysis of Single Nucleotide Polymorphisms (SNPs). Using this technique, it is possible to analyze more than 50 SNPs distributed throughout the genome in a single multiplex reaction, making it an advantage when compared with traditional sequencing reaction. In this chapter, you will find a step-by-step guide to design a multiplex primer assay for SNaPshot reaction.

Key words

SNP Genotyping Multiplex SNaPshot Primer 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Greiciane Gaburro Paneto
    • 1
    Email author
  • Francisco de Paula Careta
    • 2
  1. 1.Department of Pharmacy and NutritionFederal University of Espirito SantoAlegreBrazil
  2. 2.Department of Pharmacy and NutritionFederal University of Espirito SantoAlegreBrazil

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