Abstract
Influenza is one of the acute respiratory diseases of human caused by the influenza A (H1N1) virus and accounted for major public health concerns worldwide. The polymerase chain reaction (PCR) methods are the most popular tools for clinical diagnosis of influenza A virus. Microchip electrophoresis is a widely used method for DNA molecules separation. Herein, we describe the fast and high-throughput separation of hemagglutinin (HA) and nucleocapsid protein (NP) gene PCR products (116 bp and 195 bp, respectively) by parallel detection with multichannel microchip electrophoresis and programmed step electric field strength (PSEFS).
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Acknowledgment
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012R1A2A2A01013466).
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Zhang, P., Nan, H., Lee, S., Kang, S.H. (2015). Fast High-Throughput Screening of H1N1 Virus by Parallel Detection with Multichannel Microchip Electrophoresis. In: Van Schepdael, A. (eds) Microchip Capillary Electrophoresis Protocols. Methods in Molecular Biology, vol 1274. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2353-3_7
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DOI: https://doi.org/10.1007/978-1-4939-2353-3_7
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