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Carbon Nanotube-Based Separation Columns for Microchip Electrochromatography

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Book cover Microchip Capillary Electrophoresis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1274))

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Abstract

Fabrication of the stationary phase for microchip chromatography is most often done by packing of the individual separation channel after fabrication of the microfluidic chip, which is a very time-consuming and costly process (Kutter. J Chromatogr A 1221:72–82, 2012). Here, we describe in detail the fabrication and operation protocols for devices with microfabricated carbon nanotube stationary phases for reverse-phase chromatography. In this protocol, the lithographically defined stationary phase is fabricated in the channel before bonding of a lid, thereby circumventing the difficult packaging procedures used in more conventional protocols.

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Acknowledgment

This work was partly supported by the Danish National Advanced Technology Foundation via the project “Multiplex Blood Culture Test.”

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Authors and Affiliations

  1. Department of Micro and Nanotechnology, Technical University of Denmark, DTU, Building 345 East, 2800, Kongens Lyngby, Denmark

    K. B. Mogensen, B. Delacourt & J. P. Kutter

  2. Department of Pharmacy, University of Copenhagen Universitetsparken 2, 2100, Copenhagen, Denmark

    J. P. Kutter

Authors
  1. K. B. Mogensen
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  2. B. Delacourt
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  3. J. P. Kutter
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Corresponding author

Correspondence to J. P. Kutter .

Editor information

Editors and Affiliations

  1. Pharmaceutical Analysis, KU Leuven, Leuven, Belgium

    Ann Van Schepdael

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Mogensen, K.B., Delacourt, B., Kutter, J.P. (2015). Carbon Nanotube-Based Separation Columns for Microchip Electrochromatography. In: Van Schepdael, A. (eds) Microchip Capillary Electrophoresis Protocols. Methods in Molecular Biology, vol 1274. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2353-3_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2353-3_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2352-6

  • Online ISBN: 978-1-4939-2353-3

  • eBook Packages: Springer Protocols

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