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Structures of Glycans Bound to Receptors from Saturation Transfer Difference (STD) NMR Spectroscopy: Quantitative Analysis by Using CORCEMA-ST

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Glycoinformatics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1273))

Abstract

Glycan–receptor interactions are of fundamental relevance for a large number of biological processes, and their kinetics properties (medium/weak binding affinities) make them appropriated to be studied by ligand observed NMR techniques, among which saturation transfer difference (STD) NMR spectroscopy has been shown to be a very robust and powerful approach. The quantitative analysis of the results from a STD NMR study of a glycan–receptor interaction is essential to be able to translate the resulting spectral intensities into a 3D molecular model of the complex. This chapter describes how to carry out such a quantitative analysis by means of the Complete Relaxation and Conformational Exchange Matrix Approach for STD NMR (CORCEMA-ST), in general terms, and an example of a previous work on an antibody–glycan interaction is also shown.

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Acknowledgements

We acknowledge financial support from Spanish and Andalusian Governments (Grants CTQ2009-07168 and P07-FQM-02969, respectively), and EU (FEDER funds). P.M.E.-N. thanks Fundación Andaluza Progreso y Salud for financial support. C.G. thanks EU for a Marie Curie fellowship. J.C.M.G acknowledges CSIC for a JAE PhD fellowship, and J.A. acknowledges MICINN for a Ramon y Cajal contract. J.A. also acknowledges start-up funding from the Faculty of Science at UEA.

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Authors and Affiliations

  1. Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), C/Severo Ochoa 35, Parque Tecnológico de Andalucía, 29590, Campanillas, Málaga, Spain

    Pedro M. Enríquez-Navas

  2. Department of Bioorganic Chemistry, Instituto de Investigaciones Químicas (CSIC – US), Américo Vespucio, 49, 41092, Sevilla, Spain

    Cinzia Guzzi, Juan C. Muñoz-García, Pedro M. Nieto & Jesús Angulo

  3. School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK

    Jesús Angulo

Authors
  1. Pedro M. Enríquez-Navas
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  2. Cinzia Guzzi
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  3. Juan C. Muñoz-García
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  4. Pedro M. Nieto
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  5. Jesús Angulo
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Corresponding author

Correspondence to Jesús Angulo .

Editor information

Editors and Affiliations

  1. Justus-Liebig-University, Giessen, Germany

    Thomas Lütteke

  2. Biognos AB, Göteborg, Sweden

    Martin Frank

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Enríquez-Navas, P.M., Guzzi, C., Muñoz-García, J.C., Nieto, P.M., Angulo, J. (2015). Structures of Glycans Bound to Receptors from Saturation Transfer Difference (STD) NMR Spectroscopy: Quantitative Analysis by Using CORCEMA-ST. In: Lütteke, T., Frank, M. (eds) Glycoinformatics. Methods in Molecular Biology, vol 1273. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2343-4_28

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  • DOI: https://doi.org/10.1007/978-1-4939-2343-4_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2342-7

  • Online ISBN: 978-1-4939-2343-4

  • eBook Packages: Springer Protocols

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