Abstract
Delivery of hydrophobic compounds to photoreceptors within the retina presents unique challenges due to the anatomy and physiology of the eye. Derivatives of vitamin A (retinoids) are essential to the function and survival of photoreceptors and in the absence of an intrinsic mechanism to metabolize these compounds (visual cycle) leads to extensive loss of photoreceptors and visual function. In this chapter, we describe a method for the sustained delivery of retinoids to young mice that lack a functioning visual cycle to promote survival of photoreceptors.
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References
Tang PH, Kono M, Koutalos Y et al (2012) New insights into retinoid metabolism and cycling within the retina. Prog Retin Eye Res 32:48–63
Cascella M, Barfuss S, Stocker A (2013) Cis-retinoids and the chemistry of vision. Arch Biochem Biophys 539:187–195
Travis GH, Golczak M, Moise AR et al (2007) Diseases caused by defects in the visual cycle: retinoids as potential therapeutic agents. Annu Rev Pharmacol Toxicol 47:1–44
Znoiko SL, Rohrer B, Lu K et al (2005) Downregulation of cone-specific gene expression and degeneration of cone photoreceptors in the RPE65-/- mouse at early ages. Invest Ophthalmol Vis Sci 46:1473–1479
Tang PH, Fan J, Goletz PW et al (2010) Effective and sustained delivery of hydrophobic retinoids to photoreceptors. Invest Ophthalmol Vis Sci 51:5958–5964
Rohrer B, Lohr HR, Humphries P et al (2005) Cone opsin mislocalization in RPE65-/- mice: a defect that can be corrected by 11-cis-retinal. Invest Ophthalmol Vis Sci 46:3876–3882
Fan J, Rohrer B, Frederick JM et al (2008) RPE65-/- and LRAT-/- mice: comparable models of Leber congenital amaurosis. Invest Ophthalmol Vis Sci 49:2384–2389
Geroski DH, Edelhauser HF (2000) Drug delivery for posterior segment eye disease. Invest Ophthalmol Vis Sci 41:961–964
Cruysberg LP, Nuijts RM, Gilbert JA et al (2005) Delivery of fluorescein-labeled dexamethasone and methotrexate with fibrin sealant. Curr Eye Res 30:653–660
Hsiue GH, Chang RW, Wang CH et al (2003) Development of in situ thermosensitive drug vehicles for glaucoma therapy. Biomaterials 24:2423–2430
Miao RQ, Agata J, Chao L et al (2002) Kallistatin is a new inhibitor of angiogenesis and tumor growth. Blood 100:3245–3252
Acknowledgments
This work was supported by the National Institutes of Health Grants R01 EY04939 (R.K.C.) and CO6 RR015455 (Medical University of South Carolina); Foundation Fighting Blindness, Inc. (R.K.C.); an unrestricted grant (Department of Ophthalmology, Medical University of South Carolina) from Research to Prevent Blindness (RPB); RPB Senior Scientific Investigator Award (R.K.C.); and RPB Medical Student Research Fellowship (P.H.T.).
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Tang, P.H., Crouch, R.K. (2015). Sustained Delivery of Retinoids to Prevent Photoreceptor Death. In: Jastrzebska, B. (eds) Rhodopsin. Methods in Molecular Biology, vol 1271. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2330-4_23
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DOI: https://doi.org/10.1007/978-1-4939-2330-4_23
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2329-8
Online ISBN: 978-1-4939-2330-4
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