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Purification and Characterization of Mouse Olfactory Ensheathing Cells and Their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS)

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Immunocytochemistry and Related Techniques

Part of the book series: Neuromethods ((NM,volume 101))

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Abstract

Olfactory ensheathing cells (OECs ) are a specialized type of glia that supports axonal regeneration of olfactory neurons . OECs derived from olfactory mucosa represent a potential candidate for therapeutic use in neurological disorders , like amyotrophic lateral sclerosis (ALS ). The protocol described below produces a highly enriched population of proliferating OECs from neonatal mouse olfactory mucosa by means of an anti-Thy 1.2 antibody leading to a specific cytotoxic lysis of contaminating fibroblasts without damaging the OECs. Purified OECs expressing the two main antigenic markers, the S100 and p75 neurotrophin receptors (p75NTR ), are plated onto laminin -coated coverslips for immunofluorescence characterization , or Petri dishes for expansion and further investigations.

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Acknowledgments

The study was supported by grant #2010/20457-7, São Paulo Research Foundation (FAPESP) and CNPq, Brasilia, Brazil.

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Authors and Affiliations

  1. Neuroregeneration Center, Department of Neurology, University of São Paulo School of Medicine, São Paulo, Brazil

    Chrystian Junqueira Alves, Alexandre Baldasserini Guimarães & Jessica Ruivo Maximino

  2. Neuroregeneration Center, Department of Neurology, University of São Paulo School of Medicine, Room 2119, São Paulo, 3461, Brazil

    Gerson Chadi

  3. Laboratory of Genetics and Molecular Hematology, University of São Paulo School of Medicine, São Paulo, Brazil

    Sergio Paulo Bydlowski

Authors
  1. Chrystian Junqueira Alves
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  2. Alexandre Baldasserini Guimarães
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  3. Jessica Ruivo Maximino
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  4. Sergio Paulo Bydlowski
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  5. Gerson Chadi
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Corresponding author

Correspondence to Gerson Chadi .

Editor information

Editors and Affiliations

  1. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Adalberto Merighi

  2. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Laura Lossi

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Alves, C.J., Guimarães, A.B., Maximino, J.R., Bydlowski, S.P., Chadi, G. (2015). Purification and Characterization of Mouse Olfactory Ensheathing Cells and Their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS). In: Merighi, A., Lossi, L. (eds) Immunocytochemistry and Related Techniques. Neuromethods, vol 101. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2313-7_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2313-7_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2312-0

  • Online ISBN: 978-1-4939-2313-7

  • eBook Packages: Springer Protocols

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