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Immunocytochemical Detection of Intraneuronal Aβ Peptides in Mouse Models of Alzheimer’s Disease

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Immunocytochemistry and Related Techniques

Part of the book series: Neuromethods ((NM,volume 101))

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Abstract

Alzheimer’s disease (AD) represents a severe progressive neurodegenerative disorder and the most frequent form of dementia. It is characterized by major neuropathological hallmarks consisting of either extracellular deposited amyloid-β (Aβ) peptides or intracellular accumulations of hyperphosphorylated tau protein in the form of so-called neurofibrillary tangles (NFTs). In addition to the presence of the extracellular amyloid plaques, intraneuronal Aβ accumulations have been repeatedly reported in postmortem tissue from AD patients, as well as in numerous transgenic AD mouse models overexpressing the amyloid precursor protein (APP). Several staining protocols to detect intraneuronal Aβ exist, employing different methods of tissue pretreatment, including the use of microwave heat treatment or formic acid, among others. In this book chapter, we outline an efficient protocol for reliable antigen retrieval of intracellular Aβ in AD mouse models using paraffin-embedded brain material.

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Author information

Authors and Affiliations

  1. Department of Psychiatry and Psychotherapy, University Medical Center (UMG), Georg-August-University, von-Siebold-Str. 5, 37075, Göttingen, Germany

    Oliver Wirths & Anika Saul

Authors
  1. Oliver Wirths
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  2. Anika Saul
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Correspondence to Oliver Wirths .

Editor information

Editors and Affiliations

  1. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Adalberto Merighi

  2. Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Torino, Italy

    Laura Lossi

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Wirths, O., Saul, A. (2015). Immunocytochemical Detection of Intraneuronal Aβ Peptides in Mouse Models of Alzheimer’s Disease. In: Merighi, A., Lossi, L. (eds) Immunocytochemistry and Related Techniques. Neuromethods, vol 101. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2313-7_10

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  • DOI: https://doi.org/10.1007/978-1-4939-2313-7_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2312-0

  • Online ISBN: 978-1-4939-2313-7

  • eBook Packages: Springer Protocols

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