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Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae

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Book cover Membrane Trafficking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1270))

Abstract

Recent development of large-scale analyses such as the secretome analysis has enabled the discovery of a vast number of intracellular proteins that are secreted outside the cell. Often, these proteins do not contain any known signal sequence required for conventional protein secretion. In order to avoid misidentification of such “leaked” proteins as “secreted” proteins, reconstructing the process of protein secretion is essential. Here, we describe methods for the detection of reconstructed unconventional protein secretion and determination of regulatory proteins of secretion in Saccharomyces cerevisiae. We show that conjugating target proteins with a tag-sequence and utilizing various reagents and tools can facilitate quantitative detection of the secretion of target proteins. We expect that these methods will reveal novel unconventional secretion pathways of proteins.

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Author information

Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Natsuko Miura & Mitsuyoshi Ueda

  2. Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA

    Natsuko Miura

Authors
  1. Natsuko Miura
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  2. Mitsuyoshi Ueda
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Corresponding author

Correspondence to Mitsuyoshi Ueda .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, National University of Singapore, Singapore, Singapore

    Bor Luen Tang

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Miura, N., Ueda, M. (2015). Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae . In: Tang, B. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 1270. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2309-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2309-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2308-3

  • Online ISBN: 978-1-4939-2309-0

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