Abstract
This chapter is devoted to explain how to handle with brain specimens to get cryostat, paraffin, or floating vibratome sections and the way they are processed during ISH and IHC. The procedure to be selected is related to the study aim, general and rapid mapping, or detailed and high resolution analysis. We likewise place emphasis on how to block (embed) and prepare the tissue blocks for sectioning, insuring that optimal section planes are obtained.
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Ferran, J.L. et al. (2015). Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections. In: Hauptmann, G. (eds) In Situ Hybridization Methods. Neuromethods, vol 99. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2303-8_5
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DOI: https://doi.org/10.1007/978-1-4939-2303-8_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2302-1
Online ISBN: 978-1-4939-2303-8
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