Abstract
Surface modification of liposomes with a ligand is facilitated by the conjugation of the ligand to a hydrophobic molecule that serves to anchor the ligand to the liposomal bilayer. We describe here a simple protocol to conjugate a triphenylphosphonium group to several commercially available functionalized phospholipids. The resulting triphenylphosphonium conjugated lipids can be used to prepare liposomes that preferentially associate with mitochondria when exposed to live mammalian cells in culture.
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-2288-8_33
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Acknowledgement
This work was supported by funds from MCPHS University School of Pharmacy-Boston.
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Benein, P., Almuteri, M.A., Mehanna, A.S., D’Souza, G.G.M. (2015). Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1265. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2288-8_4
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DOI: https://doi.org/10.1007/978-1-4939-2288-8_4
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