Abstract
Trichomonas vaginalis is a sexually transmitted protozoan parasite of humans. Treatment of trichomoniasis is almost completely dependent on the old drug metronidazole and is hampered by resistance. New drug development, like routine screening for drug resistance, has however been hampered by the lack of reliable screening protocols with sufficient throughput. Here we report on two separate in vitro protocols that use fluorescent dyes and allow for standardized drug sensitivity testing on the required scale.
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Acknowledgements
This work was supported through a Research Fellowship from the Government of Saudi Arabia to M.J.N. (grant number S8306) and a Commonwealth scholarship to A.A.E.
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Natto, M.J., Eze, A.A., de Koning, H.P. (2015). Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalis . In: Hempel, J., Williams, C., Hong, C. (eds) Chemical Biology. Methods in Molecular Biology, vol 1263. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2269-7_8
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DOI: https://doi.org/10.1007/978-1-4939-2269-7_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2268-0
Online ISBN: 978-1-4939-2269-7
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