Abstract
Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequence results. Here, we describe a reliable method for the preparation of mtDNA-enriched samples from eukaryotic cells ready for direct sequencing. This protocol utilizes a conventional miniprep kit, in conjunction with a paramagnetic bead-based purification step.
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Acknowledgment
This research was supported by NIH grant P01 AG017242 (JV) and by the Ministry of Education and Science of Russian Federation grant 14.B37.21.1966 (VNP and AYM).
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Quispe-Tintaya, W., White, R.R., Popov, V.N., Vijg, J., Maslov, A.Y. (2015). Rapid Mitochondrial DNA Isolation Method for Direct Sequencing. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_9
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DOI: https://doi.org/10.1007/978-1-4939-2257-4_9
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