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Imaging Mitochondrial Hydrogen Peroxide in Living Cells

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Book cover Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1264))

Abstract

Hydrogen peroxide (H2O2) produced from mitochondria is intimately involved in human health and disease but is challenging to selectively monitor inside living systems. The fluorescent probe MitoPY1 provides a practical tool for imaging mitochondrial H2O2 and has been demonstrated to function in a variety of diverse cell types. In this chapter, we describe the synthetic preparation of the small-molecule probe MitoPY1, methods for validating this probe in vitro and in live cells, and an example procedure for measuring mitochondrial H2O2 in a cell culture model of Parkinson’s disease.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Center for Drug Discovery, Design, and Delivery, Southern Methodist University, Fondren Science Building, Office 141, 3215 Daniel Avenue, Dallas, TX, 75275, USA

    Alexander R. Lippert

  2. Department of Chemistry, The University of Chicago, IL, Chicago, USA

    Bryan C. Dickinson

  3. School of Chemistry, The University of Sydney, Sydney, NSW, Australia

    Elizabeth J. New

Authors
  1. Alexander R. Lippert
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  2. Bryan C. Dickinson
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  3. Elizabeth J. New
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Corresponding author

Correspondence to Alexander R. Lippert .

Editor information

Editors and Affiliations

  1. Midwestern University, Glendale, Arizona, USA

    Volkmar Weissig

  2. ISANH, Paris, France

    Marvin Edeas

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© 2015 Springer Science+Business Media New York

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Lippert, A.R., Dickinson, B.C., New, E.J. (2015). Imaging Mitochondrial Hydrogen Peroxide in Living Cells. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_21

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  • DOI: https://doi.org/10.1007/978-1-4939-2257-4_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2256-7

  • Online ISBN: 978-1-4939-2257-4

  • eBook Packages: Springer Protocols

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