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Mitochondrial Medicine pp 107–116Cite as

Mitochondria-Targeted RNA Import

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1264))

Abstract

The import of a modest number of nucleus-encoded RNAs into mitochondria has been reported in species ranging from yeast to human. With the advent of high-throughput RNA sequencing, additional nucleus-encoded mitochondrial RNAs are being identified. Confirming the mitochondrial localization of candidate RNAs of interest (e.g., small noncoding RNAs, miRNAs, tRNAs, and possibly lncRNAs and viral RNAs) and understanding their function within the mitochondrion is assisted by in vitro and in vivo import assay systems. Here we describe these two systems for studying mitochondrial RNA import, processing, and functions.

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References

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Acknowledgments

Supported by NIH grants F31 NS076228 (E.S.), T32 CA009120 (M.N.), R01 GM073981 (C.M.K., M.A.T.), R01 GM61721 (C.M.K.), R01 MH085683 (C.M.K.), P01 GM081621 (M.A.T.), R01 CA156674 (M.A.T.), R01 CA90571 (M.A.T.), and the Whitcome Pre-doctoral training program (E.S.)

Author information

Authors and Affiliations

  1. School of Life Sciences, Tsinghua University, Beijing, 100084, China

    Geng Wang

  2. Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA, 90095, USA

    Eriko Shimada & Carla M. Koehler

  3. Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles, MRL 4-762, 675 Charles Young Drive South, Los Angeles, CA, 90095-1732, USA

    Mahta Nili & Michael A. Teitell

  4. Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA, 90095, USA

    Carla M. Koehler & Michael A. Teitell

  5. Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, CA, 90095, USA

    Carla M. Koehler & Michael A. Teitell

  6. Broad Stem Cell Research Center, University of California at Los Angeles, Los Angeles, CA, 90095, USA

    Carla M. Koehler & Michael A. Teitell

  7. California NanoSystems Institute, University of California at Los Angeles, Los Angeles, CA, 90095, USA

    Michael A. Teitell

  8. Department of Bioengineering, University of California at Los Angeles, Los Angeles, CA, 90095, USA

    Michael A. Teitell

  9. Department of Pediatrics, University of California at Los Angeles, Los Angeles, CA, 90095, USA

    Michael A. Teitell

Authors
  1. Geng Wang
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  2. Eriko Shimada
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  3. Mahta Nili
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  4. Carla M. Koehler
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  5. Michael A. Teitell
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Corresponding author

Correspondence to Michael A. Teitell .

Editor information

Editors and Affiliations

  1. Midwestern University, Glendale, Arizona, USA

    Volkmar Weissig

  2. ISANH, Paris, France

    Marvin Edeas

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© 2015 Springer Science+Business Media New York

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Wang, G., Shimada, E., Nili, M., Koehler, C.M., Teitell, M.A. (2015). Mitochondria-Targeted RNA Import. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2257-4_11

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2256-7

  • Online ISBN: 978-1-4939-2257-4

  • eBook Packages: Springer Protocols

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