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Super-Resolution Imaging of Nuclear Bodies by STED Microscopy

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Nuclear Bodies and Noncoding RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1262))

Abstract

The sizes of nuclear bodies and other nuclear structures are normally no more than a few hundred nanometers. This size is below the resolution limit of light microscopy and thus requires electron microscopy for direct observation. Recent developments in super-resolution microscopy have extended the resolution of light microscopy to beyond 100 nm. Here, we describe a super-resolution technique, gated STED, for the analysis of the structure of nuclear bodies, with emphasis on the sample preparation and other technical tips that are important to obtain high-quality super-resolution images.

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Author information

Authors and Affiliations

  1. Laboratory for Cell Polarity Regulation, RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Osaka, 565-0824, Japan

    Yasushi Okada

  2. RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Shinichi Nakagawa

Authors
  1. Yasushi Okada
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  2. Shinichi Nakagawa
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Corresponding author

Correspondence to Yasushi Okada .

Editor information

Editors and Affiliations

  1. RNA Biology Laboratory, RIKEN, Saitama, Japan

    Shinichi Nakagawa

  2. Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan

    Tetsuro Hirose

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Okada, Y., Nakagawa, S. (2015). Super-Resolution Imaging of Nuclear Bodies by STED Microscopy. In: Nakagawa, S., Hirose, T. (eds) Nuclear Bodies and Noncoding RNAs. Methods in Molecular Biology, vol 1262. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2253-6_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2253-6_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2252-9

  • Online ISBN: 978-1-4939-2253-6

  • eBook Packages: Springer Protocols

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