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Prediction and Analysis of Intrinsically Disordered Proteins

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Structural Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1261))

Abstract

Intrinsically disordered proteins and protein regions (IDPs/IDRs) do not adopt a well-defined folded structure under physiological conditions. Instead, these proteins exist as heterogeneous and dynamical conformational ensembles. IDPs are widespread in eukaryotic proteomes and are involved in fundamental biological processes, mostly related to regulation and signaling. At the same time, disordered regions often pose significant challenges to the structure determination process, which generally requires highly homogeneous proteins samples. In this book chapter, we provide a brief overview of protein disorder, describe various bioinformatics resources that have been developed in recent years for their characterization, and give a general outline of their applications in various types of structural genomics projects. Traditionally, disordered segments were filtered out to optimize the yield of structure determination pipelines. However, it is becoming increasingly clear that the structural characterization of proteins cannot be complete without the incorporation of intrinsically disordered regions.

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Acknowledgements

This work was supported by grants of the Hungarian Scientific Research Fund (OTKA) K108798 and NK100482 [Z. D. and I. S.] and Wellcome Trust WT077044/Z/05/Z [M.P.]

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Authors and Affiliations

  1. European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK

    Marco Punta

  2. Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK

    Marco Punta

  3. Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar Tudósok körútja 2, 1117, Budapest, Hungary

    István Simon & Zsuzsanna Dosztányi

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  1. Marco Punta
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  2. István Simon
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  3. Zsuzsanna Dosztányi
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Correspondence to Zsuzsanna Dosztányi .

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  1. Oxford Protein Production Facility UK, Didcot, Oxfordshire, United Kingdom

    Raymond J. Owens

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Punta, M., Simon, I., Dosztányi, Z. (2015). Prediction and Analysis of Intrinsically Disordered Proteins. In: Owens, R. (eds) Structural Proteomics. Methods in Molecular Biology, vol 1261. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2230-7_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2230-7_3

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