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Solid-State Nuclear Magnetic Resonance Spectroscopy for Membrane Protein Structure Determination

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Structural Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1261))

Abstract

Solid-state NMR (ssNMR) is a versatile technique that can provide high-resolution (sub-angstrom) structural data for integral membrane proteins embedded in native and model membrane environments. The methodologies for a priori structure determination have for the most part been developed using samples with crystalline and fibrous morphologies. However, the techniques are now being applied to large, polytopic membrane proteins including receptors, ion channels, and porins. ssNMR data may be used to annotate and refine existing structures in regions of the protein not fully resolved by crystallography (including ligand-binding sites and mobile solvent accessible loop regions). This review describes the spectroscopic experiments and data analysis methods (including assignment) used to generate high-resolution structural data for membrane proteins. We also consider the range of sample morphologies that are appropriate for study by this method.

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Acknowledgements

The authors acknowledge funding from the Medical Research Council (UK), the Biotechnology and Biological Sciences Research Council (UK), the European Metrology Research Programme, and the National Physical Laboratory (London, UK).

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Authors and Affiliations

  1. Biomembrane Structure Unit, Biochemistry Department, South Parks Road, Oxford, OX1 3QU, UK

    Peter J. Judge, Garrick F. Taylor, Hugh R. W. Dannatt & Anthony Watts

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  1. Peter J. Judge
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  2. Garrick F. Taylor
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  3. Hugh R. W. Dannatt
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  4. Anthony Watts
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Corresponding author

Correspondence to Anthony Watts .

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Editors and Affiliations

  1. Oxford Protein Production Facility UK, Didcot, Oxfordshire, United Kingdom

    Raymond J. Owens

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Judge, P.J., Taylor, G.F., Dannatt, H.R.W., Watts, A. (2015). Solid-State Nuclear Magnetic Resonance Spectroscopy for Membrane Protein Structure Determination. In: Owens, R. (eds) Structural Proteomics. Methods in Molecular Biology, vol 1261. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2230-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-2230-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2229-1

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