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Methods for the Successful Crystallization of Membrane Proteins

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Structural Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1261))

Abstract

In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with structure determination of membrane proteins by X-ray crystallography. The advent of genomics and proteomics initiatives combined with high-throughput technologies, such as automation, miniaturization, integration, and third-generation synchrotrons, has enhanced membrane protein structure determination rate. Nevertheless, crystallization of membrane proteins still remains one of the most troublesome hurdles that every structural group must undertake. This chapter presents high-throughput methods easily available to any researcher interested in membrane protein characterization and crystallization. It is our hope this chapter can be used as a positive guide to all who are attempting crystallizing membrane proteins.

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Acknowledgments

The authors are grateful for the use of the Membrane Protein Laboratory funded by the Wellcome Trust (099165/Z/12/Z) at the Diamond Light Source. We also would like to thank to Mr. Mathew Jennions and Mr. James Birch for the fruitful discussions and all the MX beamline scientists at Diamond Light Source.

Author information

Authors and Affiliations

  1. Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK

    Isabel Moraes

  2. Membrane Protein Laboratory, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Chilton, Oxfordshire, OX11 0DE, UK

    Isabel Moraes

  3. Appleton Laboratory, Research Complex at Harwell Rutherford, Harwell, Oxford, Didcot, Oxfordshire, OX11 0FA, UK

    Isabel Moraes

  4. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB-UNL), 2780-157, Oeiras, Portugal

    Margarida Archer

Authors
  1. Isabel Moraes
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  2. Margarida Archer
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Corresponding author

Correspondence to Isabel Moraes .

Editor information

Editors and Affiliations

  1. Oxford Protein Production Facility UK, Didcot, Oxfordshire, United Kingdom

    Raymond J. Owens

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Moraes, I., Archer, M. (2015). Methods for the Successful Crystallization of Membrane Proteins. In: Owens, R. (eds) Structural Proteomics. Methods in Molecular Biology, vol 1261. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2230-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2230-7_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2229-1

  • Online ISBN: 978-1-4939-2230-7

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