Determination of RNA Chaperone Activity Using an Escherichia coli Mutant
Bacterial cold shock proteins (CSPs) function as RNA chaperones that destabilize RNA secondary structures. A quadruple mutant of Escherichia coli (cspA, cspB, cspG, and cspE) displays a cold-sensitive growth phenotype. Plant cold shock domain (CSD) proteins have been shown to complement the cold-sensitive phenotype of the E. coli mutant and to share a function with E. coli CSPs as RNA chaperones. This methodology, which is detailed here, can be utilized to reveal or probe the RNA chaperone activity of heterologous proteins.
Key wordsArabidopsis Cold shock domain (CSD) Cold shock protein (CSP) RNA chaperone Wheat
This work was supported in part by grants from the Japan Society for the Promotion of Science (KAKENHI Scientific Research B 19380063) and NARO project 112g0 (Wheat and Soybean Biotechnology) to R.I. We thank Dr. Linda Jewell for her comments on this manuscript.