In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins

  • Sarah F. MitchellEmail author
  • Roy Parker
Part of the Methods in Molecular Biology book series (MIMB, volume 1259)


mRNA binding proteins regulate gene expression by controlling the processing, localization, decay, and translation of messenger RNAs (mRNAs). To fully understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spectrometry to identify proteins that directly bind to mRNAs.

Key words

mRNA mRNA binding proteins Yeast PolyA Mass spectrometry 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of Colorado BoulderBoulderUSA
  2. 2.Howard Hughes Medical InstituteUniversity of Colorado BoulderBoulderUSA

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