Single-Molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein

  • Soraya Ait-Bara
  • Caroline Clerté
  • Emmanuel MargeatEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1259)


Single-molecule Förster Resonance Energy Transfer (smFRET) is a useful technique to probe conformational changes within bio-macromolecules. Here, we introduce how to perform smFRET measurements in solution to investigate RNA remodeling and RNA–protein interactions. In particular, we focus on how the close-to-open transition of an antiterminator hairpin is influenced by the binding of the antitermination protein and the competition by oligonucleotides.

Key words

smFRET ALEX Antitermination Hairpin Terminator Transcription Single molecules RNA 



This work was supported by the Agence Nationale de la Recherche (ANR 2010 BLAN 1525 01 to E.M.), a “Chercheur d’Avenir” grant from the Region Languedoc Roussillon to E.M., the GIS “IBiSA: Infrastructures en Biologie Sante et Agronomie” and a postdoctoral grant from the Université Montpellier I to S.A.-B.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Soraya Ait-Bara
    • 1
    • 2
    • 3
  • Caroline Clerté
    • 1
    • 2
    • 3
  • Emmanuel Margeat
    • 1
    • 2
    • 3
    Email author
  1. 1.CNRS UMR5048Centre de Biochimie StructuraleMontpellierFrance
  2. 2.INSERM U1054MontpellierFrance
  3. 3.Universités Montpellier I et IIMontpellierFrance

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