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Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production

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Insoluble Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1258))

Abstract

Despite the importance of recombinant protein production in academy and industrial fields, many issues concerning the expression of soluble and homogeneous product are still unsolved. Although several strategies were developed to overcome these obstacles, at present there is no magic bullet that can be applied for all cases. Indeed, several key expression parameters need to be evaluated for each protein. Among the different hosts for protein expression, Escherichia coli is by far the most widely used. In this chapter, we review many of the different tools employed to circumvent protein insolubility problems.

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Acknowledgments

This work was financed by a research grant from FCE-7273 and FMV-7323, 2011 from Agencia Nacional de Investigación e Innovación (ANII), Montevideo, Uruguay to P. Oppezzo. A. Correa was financed by a doctoral fellowship from ANII, Uruguay.

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  1. Recombinant Protein Unit, Institut Pasteur de Montevideo, Mataojo 2020, CP, 11400, Montevideo, Uruguay

    Agustín Correa & Pablo Oppezzo

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  1. Agustín Correa
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  2. Pablo Oppezzo
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Correspondence to Pablo Oppezzo .

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  1. Departament de Genètica i de Microbiologia, CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) and Universitat Autònoma de Barcelona, Bellaterra (Barcelona), Spain

    Elena García-Fruitós

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Correa, A., Oppezzo, P. (2015). Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production. In: García-Fruitós, E. (eds) Insoluble Proteins. Methods in Molecular Biology, vol 1258. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2205-5_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2205-5_2

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