Abstract
Purification of bacterial inclusion bodies (IBs) is gaining importance due to the raising of novel applications for this type of submicron particulate protein clusters, with potential uses in the biomedical field among others. Here, we present two optimized methods to purify IBs adapting classical procedures to the material nature as well as the requirements of its final application.
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Acknowledgment
The authors acknowledge the financial support granted to E.G.F. from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, MINECO (RTA2012-00028-C02-02), and to A.V. from Agència de Gestió d’Ajuts Universitaris i de Recerca (2009SGR-108), from MICINN (BFU2010-17450) and from the Centro de Investigación Biomédica en Red (CIBER) de Bioingeniería, Biomateriales y Nanomedicina, financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. O.C.G. received a PhD fellowship from MECD. A.V. has been distinguished with an ICREA Academia Award. S.P. acknowledges the financial support from the European Regional Development Fund (ERDF). The authors are also indebted to the Protein Production Platform (CIBER-BBN—UAB) for helpful technical assistance (http://www.bbn.ciber-bbn.es/programas/plataformas/equipamiento).
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Seras-Franzoso, J., Peternel, S., Cano-Garrido, O., Villaverde, A., García-Fruitós, E. (2015). Bacterial Inclusion Body Purification. In: García-Fruitós, E. (eds) Insoluble Proteins. Methods in Molecular Biology, vol 1258. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2205-5_16
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DOI: https://doi.org/10.1007/978-1-4939-2205-5_16
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