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Laser Scanning Microscopy in Cryobiology

  • Frank Stracke
  • Asger Kreiner-Møller
  • Heiko ZimmermannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)

Abstract

Laser scanning microscopy is emerging as a powerful imaging tool in cryobiology. The basic microscopy system can be combined with various imaging modalities including Raman spectroscopy, fluorescence correlation spectroscopy, fluorescence lifetime imaging, or multiphoton imaging. Multiphoton imaging can be used to study intracellular ice formation at the subcellular level. A Raman imaging modality can be used for chemical mapping of frozen samples. A Raman spectrum gives information about characteristic molecular vibrations of specific groups in molecules. Raman images can be used to determine the localization of intra- and extracellular constituents and the various forms of water in freeze-concentrated solutions. Spectra can be collected during freezing and thawing of a sample using a temperature-controlled sample holder. In this chapter, various advanced cryoimaging methods are described. Special emphasis is given on the different imaging modalities that can be used to study the various aspects of cryopreservation.

Key words

Cryoimaging Laser scanning microscopy Raman microspectroscopy Fluorescence imaging 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Frank Stracke
    • 1
  • Asger Kreiner-Møller
    • 1
  • Heiko Zimmermann
    • 1
    Email author
  1. 1.Fraunhofer Institute for Biomedical TechnologySt. IngbertGermany

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