Calorimetric Analysis of Cryopreservation and Freeze-Drying Formulations

  • Wendell Q. SunEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)


Differential scanning calorimetry (DSC) is a commonly used thermal analysis technique in cryopreservation and freeze-drying research. It has been used to investigate crystallization, eutectic formation, glass transition, devitrification, recrystallization, melting, polymorphism, molecular relaxation, phase separation, water transport, thermochemistry, and kinetics of complex reactions (e.g., protein denaturation). Such information can be used for the optimization of protective formulations and process protocols. This chapter gives an introduction to beginners who are less familiar with this technique. It covers the instrument and its basic principles, followed by a discussion of the methods as well as examples of specific applications.

Key words

Cryopreservation Crystallization Devitrification Differential scanning calorimeter Freeze concentration Freeze-drying Glass transition Phase separation Vitrification 


  1. 1.
    Devireddy RV, Raha D, Bischof JC (1998) Measurement of water transport during freezing in cell suspensions using a differential scanning calorimeter. Cryobiology 36:124–155CrossRefGoogle Scholar
  2. 2.
    Devireddy RV, Swanlund DJ, Roberts KP, Bischof JC (1999) Subzero water permeability parameters of mouse spermatozoa in the presence of extracellular ice and cryoprotective agents. Biol Reprod 61:764–775CrossRefGoogle Scholar
  3. 3.
    Devireddy RV, Swanlund DJ, Roberts KP, Pryor JL, Bischof JC (2000) The effect of extracellular ice and cryoprotective agents on the water permeability parameters of human sperm plasma membrane during freezing. Hum Reprod 15:1125–1135CrossRefGoogle Scholar
  4. 4.
    Sun WQ, Wagner CT, Liversey SA, Connor J (2003) Instability of frozen human erythrocytes at elevated temperatures. Cell Preserv Technol 1:255–267CrossRefGoogle Scholar
  5. 5.
    Sherlock G, Block W, Benson EE (2005) Thermal analysis of the plant encapsulation-dehydration cryopreservation protocol using silica gel as the desiccant. CryoLetters 26:45–54Google Scholar
  6. 6.
    Rall WF, Fahy GM (1985) Ice-free cryopreservation of mouse embryos at −196 degrees C by vitrification. Nature 313:573–575CrossRefGoogle Scholar
  7. 7.
    Uragami A, Sakai A, Nagai M, Takahashi T (1989) Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification. Plant Cell Rep 8:418–421CrossRefGoogle Scholar
  8. 8.
    Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Obs. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33CrossRefGoogle Scholar
  9. 9.
    Suzuki M, Tandon P, Ishikawa M, Toyomasu T (2008) Development of a new vitrification solution, VSL, and its application to the cryopreservation of gentian axillary buds. Plant Cell Rep 2:123–131Google Scholar
  10. 10.
    Sun WQ (1997) Temperature and viscosity for structural collapse and crystallization of amorphous carbohydrate solutions. CryoLetters 18:99–106Google Scholar
  11. 11.
    Kett V (2010) Development of freeze-dried formulations using thermal analysis and microscopy. Am Pharma Rev 13(6), September 1Google Scholar
  12. 12.
    Sun WQ, Davidson P (1998) Protein inactivation in amorphous sucrose and trehalose matrices: effects of phase separation and crystallization. Biochim Biophys Acta 1425:235–244CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.School of Medical Instruments and Food EngineeringUniversity of Shanghai for Science and TechnologyShanghaiChina
  2. 2.Department of Electronic Science and Technology, School of Information Science and TechnologyUniversity of Science and Technology of ChinaHefeiChina

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