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Calorimetric Analysis of Cryopreservation and Freeze-Drying Formulations

  • Wendell Q. SunEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)

Abstract

Differential scanning calorimetry (DSC) is a commonly used thermal analysis technique in cryopreservation and freeze-drying research. It has been used to investigate crystallization, eutectic formation, glass transition, devitrification, recrystallization, melting, polymorphism, molecular relaxation, phase separation, water transport, thermochemistry, and kinetics of complex reactions (e.g., protein denaturation). Such information can be used for the optimization of protective formulations and process protocols. This chapter gives an introduction to beginners who are less familiar with this technique. It covers the instrument and its basic principles, followed by a discussion of the methods as well as examples of specific applications.

Key words

Cryopreservation Crystallization Devitrification Differential scanning calorimeter Freeze concentration Freeze-drying Glass transition Phase separation Vitrification 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.School of Medical Instruments and Food EngineeringUniversity of Shanghai for Science and TechnologyShanghaiChina
  2. 2.Department of Electronic Science and Technology, School of Information Science and TechnologyUniversity of Science and Technology of ChinaHefeiChina

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