Abstract
Differential scanning calorimetry (DSC) is a commonly used thermal analysis technique in cryopreservation and freeze-drying research. It has been used to investigate crystallization, eutectic formation, glass transition, devitrification, recrystallization, melting, polymorphism, molecular relaxation, phase separation, water transport, thermochemistry, and kinetics of complex reactions (e.g., protein denaturation). Such information can be used for the optimization of protective formulations and process protocols. This chapter gives an introduction to beginners who are less familiar with this technique. It covers the instrument and its basic principles, followed by a discussion of the methods as well as examples of specific applications.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Devireddy RV, Raha D, Bischof JC (1998) Measurement of water transport during freezing in cell suspensions using a differential scanning calorimeter. Cryobiology 36:124–155
Devireddy RV, Swanlund DJ, Roberts KP, Bischof JC (1999) Subzero water permeability parameters of mouse spermatozoa in the presence of extracellular ice and cryoprotective agents. Biol Reprod 61:764–775
Devireddy RV, Swanlund DJ, Roberts KP, Pryor JL, Bischof JC (2000) The effect of extracellular ice and cryoprotective agents on the water permeability parameters of human sperm plasma membrane during freezing. Hum Reprod 15:1125–1135
Sun WQ, Wagner CT, Liversey SA, Connor J (2003) Instability of frozen human erythrocytes at elevated temperatures. Cell Preserv Technol 1:255–267
Sherlock G, Block W, Benson EE (2005) Thermal analysis of the plant encapsulation-dehydration cryopreservation protocol using silica gel as the desiccant. CryoLetters 26:45–54
Rall WF, Fahy GM (1985) Ice-free cryopreservation of mouse embryos at −196 degrees C by vitrification. Nature 313:573–575
Uragami A, Sakai A, Nagai M, Takahashi T (1989) Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification. Plant Cell Rep 8:418–421
Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Obs. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33
Suzuki M, Tandon P, Ishikawa M, Toyomasu T (2008) Development of a new vitrification solution, VSL, and its application to the cryopreservation of gentian axillary buds. Plant Cell Rep 2:123–131
Sun WQ (1997) Temperature and viscosity for structural collapse and crystallization of amorphous carbohydrate solutions. CryoLetters 18:99–106
Kett V (2010) Development of freeze-dried formulations using thermal analysis and microscopy. Am Pharma Rev 13(6), September 1
Sun WQ, Davidson P (1998) Protein inactivation in amorphous sucrose and trehalose matrices: effects of phase separation and crystallization. Biochim Biophys Acta 1425:235–244
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Sun, W.Q. (2015). Calorimetric Analysis of Cryopreservation and Freeze-Drying Formulations. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_6
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2193-5_6
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-2192-8
Online ISBN: 978-1-4939-2193-5
eBook Packages: Springer Protocols