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Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cells

  • Willem F. WolkersEmail author
  • Harriëtte Oldenhof
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)

Abstract

An infrared spectrum gives information about characteristic molecular vibrations of specific groups in molecules. Fourier transform infrared spectroscopy can be applied to study lipids and proteins in cells or tissues. Spectra can be collected during cooling, heating, or dehydration of a sample using a temperature-controlled sample holder or a sample holder for controlled dehydration. In the current chapter, acquisition and analysis of infrared spectra during cooling, warming, or dehydration is described. Spectra analysis involving assessment of specific band positions, areas, or ratios is described. Special emphasis is given on studying membrane phase behavior and protein denaturation in cells or tissues. In addition, methods are presented to determine the water-to-ice phase change during freezing, dehydration kinetics, and the glass transition temperature of amorphous systems.

Key words

Dehydration Freeze-drying Freezing Fourier transform infrared spectroscopy (FTIR) Membrane phase behavior Protein denaturation 

Notes

Acknowledgment

This work is supported by funding from the German Research Foundation (DFG, Deutsche Forschungsgemeinschaft) for the Cluster of Excellence “From Regenerative Biology to Reconstructive Therapy” (REBIRTH).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institute of Multiphase ProcessesLeibniz Universität HannoverHannoverGermany
  2. 2.Unit for Reproductive Medicine, Clinic for HorsesUniversity of Veterinary Medicine HannoverHannoverGermany

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