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Freeze-Drying of Mammalian Sperm

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Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1257))

Abstract

Long-term preservation of mammalian sperm at suprazero temperatures is desired to save storage and space costs as well as to facilitate transport of preserved samples. This can be accomplished by the freeze-drying of sperm samples. Although freeze-drying results in immotile and membrane-compromised sperm, intracytoplasmic sperm injection (ICSI) can be used to introduce such an immotile sperm into an oocyte and thus start the fertilization process. So far, it has been shown that improved freeze-drying protocols preserve chromosomal integrity and oocyte-activating factor(s) at 4 °C for several years and at ambient temperature for approximately 1 month, which permits shipping freeze-dried samples at ambient temperature. This chapter concisely reviews freeze-drying of mammalian sperm first and then presents a simple freeze-drying protocol.

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Author information

Authors and Affiliations

  1. School of Medicine, Sher Institute for Reproductive Medicine and University of Nevada, Las Vegas, NV, USA

    Levent Keskintepe

  2. Department of Neuroscience and Regenerative Medicine; Department of Medicine, Medical College of Georgia, Georgia Regents University, 1120 15th Street, Augusta, GA, 30912, USA

    Ali Eroglu Ph.D.

  3. Department of Obstetrics and Gynecology, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA

    Ali Eroglu Ph.D.

Authors
  1. Levent Keskintepe
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  2. Ali Eroglu Ph.D.
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Corresponding author

Correspondence to Ali Eroglu Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany

    Willem F. Wolkers

  2. Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

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Keskintepe, L., Eroglu, A. (2015). Freeze-Drying of Mammalian Sperm. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_25

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  • DOI: https://doi.org/10.1007/978-1-4939-2193-5_25

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-2192-8

  • Online ISBN: 978-1-4939-2193-5

  • eBook Packages: Springer Protocols

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