Freeze-Drying of Proteins

  • Baolin LiuEmail author
  • Xinli Zhou
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)


Freeze-drying has become one of the most important processes for the preservation of biological products. This chapter provides protocols for freeze-drying of proteins and discusses the importance of formulation, cycle development, and validation. Specific formulations for stabilization of proteins are presented as well as advice on common problems with freeze-drying of proteins.

Key words

Freeze-drying Proteins Formulation Stabilizer Lyophilization 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.School of Medical Instrument and Food EngineeringInstitute of Biothermal ScienceShanghaiChina

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