Abstract
Plant cell cultures may consist of dedifferentiated cells as well as of cells showing embryogenic potential. They can be used for very different purposes in research and biotechnology as well as for plant propagation. For such cell cultures, cryopreservation is the only means for long-term preservation. Most of the different cryopreservation approaches, which are generally used for plant tissues, have also been applied to plant cell cultures; they include slow freezing, vitrification, and encapsulation/dehydration approaches. The controlled-rate slow freezing approach which is described here, however, remains to be the gold standard for cell cultures. In this chapter, a standard cryopreservation procedure is presented for plant cell cultures.
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References
Tabata H (2004) Paclitaxel production by plant cell culture technology. Adv Biochem Eng Biotechnol 87:1–23
Hellwig S, Drossard J, Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant proteins. Nat Biotechnol 11:1415–1422
Ziv M (2005) Simple bioreactors for mass propagation of plants. In: Hvoslef-Eide AK, Preil W (eds) Liquid culture systems for in-vitro plant propagation. Springer, Berlin, pp 79–93
Toshiyuki N, Seichiro H, Inzé D (2010) Tobacco BY2 cells. In: Lörz H, Widholm J (eds) Biotechnology in agriculture and forestry. Springer, Berlin
Kim KW, Bamba T, Harada K, Fukusaki E, Kobayashi A (2006) Time course of metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment. J Exp Bot 58:415–424
Withers L, King P (1980) A simple freezing unit and routine cryopreservation method for plant cell cultures. Cryo Letters 1:213–220
Hao YJ, You CX, Deng XX (2002) Effects of cryopreservation on developmental competency, cytological and molecular stability of citrus callus. Cryo Letters 23:27–35
Van Eck JV, Keen P (2009) Continued expression of plant-made vaccines following long-term cryopreservation of antigen-expressing tobacco cell cultures. In Vitro Cell Dev Biol Plant 45:750–757
Shibli RA, Haagenson DM, Cunningham SM, Berg WK, Volenec JJ (2001) Cryopreservation of alfalfa (Medicago sativa L.) by encapsulation–dehydration. Plant Cell Rep 20:445–450
Bachiri Y, Bajon C, Sauvant A, Gazeau C, Morisset C (2000) Effect of osmotic stress tolerance of air-drying and cryopreservation of Arabidopsis thaliana suspension cells. Protoplasma 214:227–243
Ogawa Y, Suzuki H, Sakurai N, Akoi K, Shibata D (2008) Cryopreservation and metabolic profiling analysis of Arabidopsis thaliana T87 suspension cultures cells. Cryo Letters 29:427–436
Keller EJR, Senula A, Höfer M, Heine-Dobbernack E, Schumacher HM (2013) Cryopreservation of plant cells. In: Flickinger MC (ed) Encyclopedia of industrial biotechnology: bioprocess, bioseparation and cell technology. John Wiley & Sons, (2013), New Jersey 1–14
Lynch PT, Benson EE, Jones J, Cocking EC, Power JB, Davey MR (1994) Rice cell cryopreservation: the influence of culture methods and the embryogenic potential of cell suspension on the post-thaw recovery. Plant Sci 98:185–192
Fki L, Bouaziz N, Sahnoun N, Swennen R, Dir N, Panis B (2011) Palm cryobanking. Cryo Letters 32:451–462
González-Benito ME, Martin C, Vidal JR (2009) Cryopreservation of embryogenic cell suspensions of the Spanish grapevine cultivars ‘Albarino’ and ‘Tempranillo’. Vitis 48:131–136
Mikula A, Olas M, Sliwinska E, Rybczynski JJ (2008) Cryopreservation by encapsulation of Gentiana ssp.: cell suspensions maintains regrowth, embryogenic competence and DNA content. Cryo Letters 29:409–418
Reinhoud PJ, Uragami S, Sakai A, van Iren F (1995) Vitrification of plant cell suspensions. In: Day JG, McClellan MR (eds) Cryopreservation and freeze-drying protocols. Humana Press, Totowa, NJ, p 113–120
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Schumacher, H.M., Westphal, M., Heine-Dobbernack, E. (2015). Cryopreservation of Plant Cell Lines. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_21
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DOI: https://doi.org/10.1007/978-1-4939-2193-5_21
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