Cord Blood Clinical Processing, Cryopreservation, and Storage

  • Heidi Elmoazzen
  • Jelena L. HolovatiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)


Allogeneic umbilical cord blood (UCB) hematopoietic stem cell transplantation has become a crucial advancement in the treatment for a variety of diseases including hematopoietic and non-hematopoietic malignancies, BM failure syndromes, hemoglobinopathies, and metabolic and immunodeficiency disorders. It has been well documented that the success of UCB engraftment is tied to UCB banking processes, and now there are established guidelines for standardization of collection, banking, processing, and cryopreservation for unrelated UCB units with purpose of achieving consistent production of high quality placental and UCB units for administration. In 2011, Canada’s Ministry of Health has announced Canada’s first national, publicly funded umbilical cord blood bank, which aims to provide altruistic donations for unrelated allogeneic hematopoietic stem cell transplant. In this chapter, we describe specific protocols for clinical processing, cryopreservation, and storage of UCB used by the Canadian Blood Services National Public Umbilical Cord Blood Bank.

Key words

Umbilical cord blood (UCB) Hematopoietic stem cells (HSC) Transplant Cryopreservation Processing Cord blood bank 



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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Canadian Blood Services National Public Cord Blood BankOttawaCanada
  2. 2.Canadian Blood Services Centre for InnovationEdmontonCanada
  3. 3.Department of Laboratory Medicine and PathologyUniversity of Alberta, Edmonton Clinic Health Academy 5-407EdmontonCanada

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