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Cryopreservation and Freeze-Drying Protocols pp 369–379Cite as

Cord Blood Clinical Processing, Cryopreservation, and Storage

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1257))

Abstract

Allogeneic umbilical cord blood (UCB) hematopoietic stem cell transplantation has become a crucial advancement in the treatment for a variety of diseases including hematopoietic and non-hematopoietic malignancies, BM failure syndromes, hemoglobinopathies, and metabolic and immunodeficiency disorders. It has been well documented that the success of UCB engraftment is tied to UCB banking processes, and now there are established guidelines for standardization of collection, banking, processing, and cryopreservation for unrelated UCB units with purpose of achieving consistent production of high quality placental and UCB units for administration. In 2011, Canada’s Ministry of Health has announced Canada’s first national, publicly funded umbilical cord blood bank, which aims to provide altruistic donations for unrelated allogeneic hematopoietic stem cell transplant. In this chapter, we describe specific protocols for clinical processing, cryopreservation, and storage of UCB used by the Canadian Blood Services National Public Umbilical Cord Blood Bank.

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Author information

Authors and Affiliations

  1. Canadian Blood Services National Public Cord Blood Bank, Ottawa, ON, Canada

    Heidi Elmoazzen

  2. Canadian Blood Services Centre for Innovation, Edmonton, AB, Canada

    Jelena L. Holovati Ph.D.

  3. Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton Clinic Health Academy 5-407, 11405-87 Av., Edmonton, AB, Canada, T6G 1C9

    Jelena L. Holovati Ph.D.

Authors
  1. Heidi Elmoazzen
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  2. Jelena L. Holovati Ph.D.
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Corresponding author

Correspondence to Jelena L. Holovati Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany

    Willem F. Wolkers

  2. Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

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Disclaimer

This publication is provided without any representations or warranties of any kind, whether express or implied, regarding the completeness, accuracy, reliability, or suitability of the information contained herein or its fitness for a particular purpose or use. None of Canadian Blood Services, the authors or other persons involved in the creation of this manuscript shall be responsible for the consequences of any action taken on the basis of the information contained in this publication, or any errors or omissions therefrom. There are no conflicts of interest from the authors of this publication.

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Elmoazzen, H., Holovati, J.L. (2015). Cord Blood Clinical Processing, Cryopreservation, and Storage. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-2193-5_18

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-2192-8

  • Online ISBN: 978-1-4939-2193-5

  • eBook Packages: Springer Protocols

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