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Cryopreservation and Freeze-Drying Protocols pp 343–352Cite as

Sperm Cleanup and Centrifugation Processing for Cryopreservation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1257))

Abstract

Fertility rates with artificial insemination are highest with good-quality sperm samples. Therefore, nonviable sperm, cellular debris, and seminal plasma are preferably removed from semen samples prior to use or for preservation. Such compounds are sources where reactive oxygen species are generated during storage or upon cryopreservation, impairing sperm function. In this chapter we describe methods to remove seminal plasma and cellular debris from sperm samples, and for selecting morphologically normal motile sperm. The methods that are described here include: ordinary centrifugation, sperm swim-up, glass wool and Sephadex filtration/adherence, and single-layer as well as discontinuous two-layer iodixanol density gradient centrifugation.

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Author information

Authors and Affiliations

  1. Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Bünteweg 15, 30559, Hannover, Germany

    Harald Sieme & Harriëtte Oldenhof

Authors
  1. Harald Sieme
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  2. Harriëtte Oldenhof
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Corresponding author

Correspondence to Harald Sieme .

Editor information

Editors and Affiliations

  1. Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany

    Willem F. Wolkers

  2. Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

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Sieme, H., Oldenhof, H. (2015). Sperm Cleanup and Centrifugation Processing for Cryopreservation. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_16

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  • DOI: https://doi.org/10.1007/978-1-4939-2193-5_16

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-2192-8

  • Online ISBN: 978-1-4939-2193-5

  • eBook Packages: Springer Protocols

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