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Membrane Modification Strategies for Cryopreservation

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Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1257))

Abstract

Cell membranes can be modified using cyclodextrins loaded with lipids or unilamellar liposomes. Lipid choice can greatly influence the organization of the targeted membrane and result in a cell that is more capable of surviving cryopreservation due to altered membrane-phase transition properties or membrane reorganization that may alter the normal physiologic processes of the treated cell. The protocols described here explain the preparation of the cyclodextrins and liposomes, impact of the amount and type of lipids, and general principles for treating cells using either of these technologies.

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Author information

Authors and Affiliations

  1. United States Department of Agriculture, Agricultural Research Service, National Animal Germplasm Program, National Center for Genetic Resources Preservation, 1111 S. Mason St., Fort Collins, CO, 80521-4500, USA

    Phillip H. Purdy Ph.D.

  2. Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA

    James K. Graham

Authors
  1. Phillip H. Purdy Ph.D.
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  2. James K. Graham
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Corresponding author

Correspondence to Phillip H. Purdy Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany

    Willem F. Wolkers

  2. Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

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Purdy, P.H., Graham, J.K. (2015). Membrane Modification Strategies for Cryopreservation. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-2193-5_15

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-2192-8

  • Online ISBN: 978-1-4939-2193-5

  • eBook Packages: Springer Protocols

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