Advertisement

Membrane Modification Strategies for Cryopreservation

  • Phillip H. PurdyEmail author
  • James K. Graham
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)

Abstract

Cell membranes can be modified using cyclodextrins loaded with lipids or unilamellar liposomes. Lipid choice can greatly influence the organization of the targeted membrane and result in a cell that is more capable of surviving cryopreservation due to altered membrane-phase transition properties or membrane reorganization that may alter the normal physiologic processes of the treated cell. The protocols described here explain the preparation of the cyclodextrins and liposomes, impact of the amount and type of lipids, and general principles for treating cells using either of these technologies.

Key words

Cyclodextrin Liposome Lipid Cryopreservation 

References

  1. 1.
    Parks JE, Lynch DV (1992) Lipid composition and thermotropic phase behavior of boar, bull, stallion and rooster sperm membranes. Cryobiology 29:255–266CrossRefGoogle Scholar
  2. 2.
    He L, Bailey JL, Buhr MM (2001) Incorporating lipids into boar sperm decreases chilling sensitivity but not capacitation potential. Biol Reprod 64:69–79CrossRefGoogle Scholar
  3. 3.
    Tomás C, Blanch E, Fazeli A, Mocé E (2013) Effect of a pre-freezing treatment with cholesterol-loaded cyclodextrins on boar sperm longevity, capacitation dynamics, ability to adhere to porcine oviductal epithelial cells in vitro and DNA fragmentation dynamics. Reprod Fert Dev 25:935–946CrossRefGoogle Scholar
  4. 4.
    Tomás C, Blanch E, Cebrián B, Mocé E (2013) In vivo fertilising ability of frozen-thawed boar sperm treated with cholesterol-loaded cyclodextrins prior to cryopreservation. Anim Reprod Sci 140:77–82CrossRefGoogle Scholar
  5. 5.
    Konyali C, Tomás C, Blanch E, Gómez EA, Graham JK, Mocé E (2013) Optimizing conditions for treating goat semen with cholesterol-loaded cyclodextrins prior to freezing to improve cryosurvival. Cryobiology 67:124–131CrossRefGoogle Scholar
  6. 6.
    Farshad A, Amidi F, Khor AK, Rashidi A (2011) Effect of cholesterol-loaded-cyclodextrin in presence and absence of egg yolk during freezing step on quality of markhoz buck’s spermatozoa. Asian-Aust J Anim Sci 24:181–189CrossRefGoogle Scholar
  7. 7.
    Purdy PH, Graham JK (2004) Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology 48:36–45CrossRefGoogle Scholar
  8. 8.
    Graham JK, Foote RH (1987) Effect of several lipids, fatty acyl chain length, and degree of unsaturation on the motility of bull spermatozoa after cold shock and freezing. Cryobiology 24:42–52CrossRefGoogle Scholar
  9. 9.
    Moraes EA, Graham JK, Torres CAA, Meyers M, Spizziri B (2010) Delivering cholesterol or cholestanol to bull sperm membranes improves cryosurvival. Anim Reprod Sci 118:148–154CrossRefGoogle Scholar
  10. 10.
    Röpke T, Oldenhof H, Leiding C, Sieme H, Bollwein H, Wolkers WF (2011) Liposomes for cryopreservation of bovine sperm. Theriogenology 76:1465–1472CrossRefGoogle Scholar
  11. 11.
    Holt WV, North RD (1988) The role of membrane-active lipids in the protection of ram spermatozoa during cooling and storage. Gamete Res 19:77–89CrossRefGoogle Scholar
  12. 12.
    Mocé E, Purdy PH, Graham JK (2010) Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival. Anim Reprod Sci 118:236–247CrossRefGoogle Scholar
  13. 13.
    Motamedi-Mojdehi R, Roostaei-Ali Mehr M, Rajabi-Toustani R (2014) Effect of different levels of glycerol and cholesterol-loaded cyclodextrin on cryosurvival of ram spermatozoa. Reprod Dom Anim 49:65–70CrossRefGoogle Scholar
  14. 14.
    Klein U, Gimpl G, Fahrenholz F (1995) Alteration of the myometrial plasma membrane cholesterol content with β-cyclodextrin modulates the binding affinity of the oxytocin receptor. Biochemistry 34:13784–13793CrossRefGoogle Scholar
  15. 15.
    Wilhelm KM, Graham JK, Squires EL (1996) Effects of phosphatidylserine and cholesterol liposomes on the viability, motility, and acrosomal integrity of stallion spermatozoa prior to and after cryopreservation. Cryobiology 33:320–329CrossRefGoogle Scholar
  16. 16.
    Pillet E, Labbe C, Batellier F, Duchamp G, Beaumal V, Anton M, Desherce S, Schmitt E, Magistrini M (2012) Liposomes as an alternative to egg yolk in stallion freezing extender. Theriogenology 77:268–279CrossRefGoogle Scholar
  17. 17.
    Oldenhof H, Gojowsky M, Wang S, Henke S, Yu C, Rohn K, Wolkers WF, Sieme H (2013) Osmotic stress and membrane phase changes during freezing of stallion sperm: mode of action of cryoprotective agents. Biol Reprod 88:68CrossRefGoogle Scholar
  18. 18.
    He Q, Lu G, Che K, Zhao E, Fang Q, Wang H, Liu J, Huang C, Dong Q (2011) Sperm cryopreservation of the endangered red spotted grouper, Epinephelus akaara, with a special emphasis on membrane lipids. Aquaculture 318:185–190CrossRefGoogle Scholar
  19. 19.
    Kiso WK, Asano A, Travis AJ, Schmitt DL, Brown JL, Pukazhenthi BS (2012) Pretreatment of Asian elephant (Elephas maximus) spermatozoa with cholesterol-loaded cyclodextrins and glycerol addition at 4 °C improves cryosurvival. Reprod Fert Dev 24:1134–1142CrossRefGoogle Scholar
  20. 20.
    Hussain SA, Lessard C, Anzar M (2013) A strategy for improvement of postthaw quality of bison sperm. Theriogenology 79:108–115CrossRefGoogle Scholar
  21. 21.
    Zeron Y, Tomczak M, Crowe J, Arav A (2002) The effect of liposomes on thermotropic membrane phase transitions of bovine spermatozoa and oocytes: implications for reducing chilling sensitivity. Cryobiology 45:143–152CrossRefGoogle Scholar
  22. 22.
    Horvath G, Seidel GE Jr (2006) Vitrification of bovine oocytes after treatment with cholesterol-loaded methyl-β-cyclodextrin. Theriogenology 66:1026–1033CrossRefGoogle Scholar
  23. 23.
    Stadnick H, Stoll C, Wolkers WF, Acker JP, Holovati JL (2011) The effect of liposome treatment on the quality of hypothermically stored red blood cells. Biopreserv Biobank 9:335–342CrossRefGoogle Scholar
  24. 24.
    Stoll C, Holovati JL, Acker JP, Wolkers WF (2012) Synergistic effects of liposomes, trehalose, and hydroxyethyl starch for cryopreservation of human erythrocytes. Biotechnol Prog 28:364–371CrossRefGoogle Scholar
  25. 25.
    Graham JK, Foote RH, Parrish JJ (1986) Effect of dilauroylphosphatidylcholine on the acrosome reaction and subsequent penetration of bull spermatozoa into zona-free hamster eggs. Biol Reprod 35:413–424CrossRefGoogle Scholar
  26. 26.
    Amorim EAM, Graham JK, Spizziri B, Meyers M, Torres CAA (2009) Effect of cholesterol or cholesteryl conjugates on the cryosurvival of bull sperm. Cryobiology 58:210–214CrossRefGoogle Scholar
  27. 27.
    Purdy PH, Graham JK (2004) Effect of adding cholesterol to bull sperm membranes on sperm capacitation, the acrosome reaction, and fertility. Biol Reprod 71:522–527CrossRefGoogle Scholar
  28. 28.
    Nolan JP, Graham JK, Hammerstedt RH (1992) Artificial induction of exocytosis in bull sperm. Arch Biochem Biophys 292:311–322CrossRefGoogle Scholar
  29. 29.
    Hope MJ, Bally MB, Webb G, Cullis PR (1985) Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to maintain a membrane potential. Biochem Biophys Acta 812:55–65CrossRefGoogle Scholar
  30. 30.
    Stewart JCM (1980) Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Anal Biochem 104:10–14CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.United States Department of Agriculture, Agricultural Research Service, National Animal Germplasm ProgramNational Center for Genetic Resources PreservationFort CollinsUSA
  2. 2.Department of Biomedical SciencesColorado State UniversityFort CollinsUSA

Personalised recommendations