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Cryopreservation of Greenshell™ Mussel (Perna canaliculus) Sperm

  • Serean L. AdamsEmail author
  • John F. Smith
  • Jolene Taylor
  • Lindsay T. McGowan
  • H. Robin Tervit
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)

Abstract

Cryopreservation is a valuable technique for aquaculture as it enables a library or bank of genetically valuable animals to be maintained in a cost-effective manner. Here, we describe a method to cryopreserve the sperm of the Greenshell™ mussel (Perna canaliculus) and how to use the sperm post-thawing to maximize larval production from thawed sperm in selective breeding.

Key words

Sperm, Greenshell™ mussel, Cryopreservation, Fertility, Larvae 

Notes

Acknowledgment

This research was funded by the New Zealand Ministry of Business, Innovation, and Employment (CAWX0802 and CAWX1315). We thank SPATnz and Kono for supplying mussels and technical assistance and also thank the staff of Cawthron Aquaculture Park (in particular Jonathan Morrish and Ellie Watts) for technical assistance.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Serean L. Adams
    • 1
    Email author
  • John F. Smith
    • 1
  • Jolene Taylor
    • 1
  • Lindsay T. McGowan
    • 2
  • H. Robin Tervit
    • 1
  1. 1.Cawthron InstituteNelsonNew Zealand
  2. 2.AgResearch Ltd.HamiltonNew Zealand

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