Skip to main content
SpringerLink
Log in

Cryopreservation of Greenshell™ Mussel (Perna canaliculus) Sperm

  • Protocol
  • First Online:
Cryopreservation and Freeze-Drying Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1257))

Abstract

Cryopreservation is a valuable technique for aquaculture as it enables a library or bank of genetically valuable animals to be maintained in a cost-effective manner. Here, we describe a method to cryopreserve the sperm of the Greenshell™ mussel (Perna canaliculus) and how to use the sperm post-thawing to maximize larval production from thawed sperm in selective breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. McFadzen IRB (1995) Cryopreservation of sperm of the Pacific oyster Crassostrea gigas. Methods Mol Bio 38:145–149

    CAS  Google Scholar 

  2. Adams SL, Smith JF, Roberts RD, Jankec AR, Kaspar HF, Tervit HR, Pugh PA, Webb SC, King NG (2004) Cryopreservation of sperm of the Pacific oyster (Crassostrea gigas): development of a practical method for commercial spat production. Aquaculture 242:271–282

    Article  CAS  Google Scholar 

  3. Tervit HR, Adams SL, Roberts RD, McGowan LT, Pugh PA, Smith JF, Janke AR (2005) Successful cryopreservation of Pacific oyster (Crassostrea gigas) oocytes. Cryobiology 51:142–151

    Article  CAS  Google Scholar 

  4. Tiersch TR, Yang H, Jenkins JA, Dong Q (2007) Sperm cryopreservation in fish and shellfish. Soc Reprod Fertil Suppl 65:493–508

    Google Scholar 

  5. Adams SL, Smith JF, Roberts RD, Janke AR, King NG, Tervit HR, Webb SC (2008) Application of sperm cryopreservation in selective breeding of the Pacific oyster, Crassostrea gigas (Thunberg). Aquacult Res 39:1434–1442

    Article  Google Scholar 

  6. Environment Agency UK (2007) The direct toxicity assessment of aqueous environmental samples using the oyster (Crassostrea gigas) embryo-larval development test: methods for the examination of waters and associated materials, Environment Agency UK, Bristol, UK. http://www.environment-agency.gov.uk/static/documents/Research/oyster209jan30_1388168.pdf. Accessed 26 Feb 2014.

  7. Environment Canada (2011) Biological test method: fertilization assay using echinoids (Sea Urchins and Sand Dollars) EPS 1/RM/27, 2nd ed, Ottawa, Canada. http://www.ec.gc.ca/Publications/047B08AB-530E-49EA-8EC8-8AB8E8D75DA4/1_BiologicalTestMethodFertilizationAsayUsingEchinoidsSeaUrchinsSandDollars2ndEdition.pdf. Accessed 26 Feb 2014.

  8. ASTM (2012) E724: standard guide for conducting static acute toxicity tests starting with embryos of four species of saltwater bivalve molluscs. ASTM International, West Conshohocken, PA

    Google Scholar 

  9. ASTM (2012) E1563: standard guide for conducting static acute toxicity tests with echinoid embryos. ASTM International, West Conshohocken, PA

    Google Scholar 

  10. Paredes E, Bellas J (2009) Cryopreservation of sea urchin embryos (Paracentrotus lividus) applied to marine ecotoxicological studies. Cryobiology 59:344–350

    Article  CAS  Google Scholar 

  11. Paniagua-Chavez CG, Tiersch TR (2001) Laboratory studies of cryopreservation of sperm and trochophore larvae of the eastern oyster. Cryobiology 43:211–223

    Article  CAS  Google Scholar 

  12. Adams SL, Hessian PA, Mladenov PV (2004) Cryopreservation of sea urchin (Evechinus chloroticus) sperm. Cryo Letters 25:287–299

    Google Scholar 

  13. Dong Q, Eudeline B, Huang C, Allen SK Jr, Tiersch TR (2005) Commercial-scale sperm cryopreservation of diploid and tetraploid Pacific oysters, Crassostrea gigas. Cryobiology 50:1–16

    Article  Google Scholar 

  14. Adams SL, Smith JF, Tervit HR, McGowan LT, Roberts RD, Janke AR, King NG, Gale SL, Webb SC (2011) Cryopreservation of molluscan sperm: Pacific oyster, Green-lipped mussel and Paua abalone. In: Tiersch TR, Green CC (eds) Cryopreservation in aquatic species, 2nd edn. World Aquaculture Society, Baton Rouge, LA, pp 562–573

    Google Scholar 

  15. Smith JF, Adams SL, Gale SL, McGowan LT, Tervita HR, Roberts RD (2012) Cryopreservation of Greenshell™ mussel (Perna canaliculus) sperm. I. Establishment of freezing protocol. Aquaculture 334–337:199–204

    Article  Google Scholar 

  16. Paredes E, Adams SL, Tervit HR, Smith JF, McGowan LT, Gale SL, Morrish JR, Watts E (2012) Cryopreservation of Greenshell™ mussel (Perna canaliculus) trochophore larvae. Cryobiology 65:256–262

    Article  CAS  Google Scholar 

  17. Paredes E, Bellas J, Adams SL (2013) Comparative cryopreservation study on two species of bivalves: Pacific oyster (Crassostrea gigas) and blue mussel (Mytilus galloprovincialis). Cryobiology 67:274–279

    Article  CAS  Google Scholar 

  18. Liu Y, Xu T, Robinson N, Qin J, Li X (2014) Cryopreservation of sperm in farmed Australian greenlip abalone Haliotis laevigata. Cryobiology 68:185–193

    Article  Google Scholar 

  19. Aquaculture New Zealand (2012) New Zealand aquaculture: a sector overview with key facts, statistics and trends. http://aquaculture.org.nz/wp-content/uploads/2012/05/NZ-Aquaculture-Facts-2012.pdf. Accessed 13 Dec 2013.

  20. Alfaro AC, Jeffs AG, Hooker SH (2001) Reproductive behaviour of the green-lipped mussel, Perna canaliculus, in northern New Zealand. Bull Mar Sci 69:1095–1108

    Google Scholar 

  21. Martinez-Pastor F, Adams SL (2008) Cryobiological material and handling procedures. In: Cabrita E, Robels V, Herraez P (eds) Methods in reproductive aquaculture: marine and freshwater species. CRC, Boca Raton, FL, pp 295–319

    Chapter  Google Scholar 

  22. Adams SL, Tervit HR, McGowan LT, Smith JF, Roberts RD, Salinas-Flores L, Gale SL, Webb SC, Mullen SF, Critser JK (2009) Towards cryopreservation of Greenshell mussel (Perna canaliculus) oocytes. Cryobiology 58:69–74

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This research was funded by the New Zealand Ministry of Business, Innovation, and Employment (CAWX0802 and CAWX1315). We thank SPATnz and Kono for supplying mussels and technical assistance and also thank the staff of Cawthron Aquaculture Park (in particular Jonathan Morrish and Ellie Watts) for technical assistance.

Author information

Authors and Affiliations

  1. Cawthron Institute, 98 Halifax Street East, Private Bag 2, Nelson, 7042, New Zealand

    Serean L. Adams Ph.D., John F. Smith, Jolene Taylor & H. Robin Tervit

  2. AgResearch Ltd., Private Bag 3123, Hamilton, 3240, New Zealand

    Lindsay T. McGowan

Authors
  1. Serean L. Adams Ph.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John F. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jolene Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lindsay T. McGowan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Robin Tervit
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Serean L. Adams Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, Germany

    Willem F. Wolkers

  2. Unit for Reproductive Medicine, Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany

    Harriëtte Oldenhof

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Adams, S.L., Smith, J.F., Taylor, J., McGowan, L.T., Tervit, H.R. (2015). Cryopreservation of Greenshell™ Mussel (Perna canaliculus) Sperm. In: Wolkers, W., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 1257. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2193-5_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-2193-5_14

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-2192-8

  • Online ISBN: 978-1-4939-2193-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation