Efficient Cryopreservation of Human Pluripotent Stem Cells by Surface-Based Vitrification

  • Julia C. Neubauer
  • Axel F. Beier
  • Niels Geijsen
  • Heiko ZimmermannEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1257)


Efficient cryopreservation of human stem cells is crucial for guaranteeing a permanent supply of high-quality cell material for drug discovery or regenerative medicine. Conventionally used protocols usually employing slow freezing rates, however, result in low recovery rates for human pluripotent stem cells due to their complex colony structure. In this chapter, a surface-based vitrification protocol for pluripotent stem cells is presented based on a procedure for human embryonic stem cells developed by Beier et al. (Cryobiology 63:175–185, 2011). This simple and highly efficient cryopreservation method allows cryopreservation of large numbers of ready-to-use adherent cells that maintain pluripotency.

Key words

Cryopreservation Pluripotent stem cells Surface-based vitrification Vitrification 



The work with human embryonic stem cells was permitted by the Robert Koch Institute (18 and 44 permission) and carried out according to German law.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Julia C. Neubauer
    • 1
  • Axel F. Beier
    • 1
    • 2
  • Niels Geijsen
    • 2
    • 3
  • Heiko Zimmermann
    • 1
    • 4
    Email author
  1. 1.Fraunhofer Institute for Biomedical Technology (IBMT)St. IngbertGermany
  2. 2.Hubrecht Institute and University Medical Center UtrechtUtrechtNetherlands
  3. 3.Utrecht University School for Veterinary MedicineUtrechtNetherlands
  4. 4.Saarland UniversitySaarbrückenGermany

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